Studying MHC class II Presentation of Immobilized Antigen by B Lymphocytes

  • M. I. Yuseff
  • A. M. Lennon-DumenilEmail author
Part of the Methods in Molecular Biology™ book series (MIMB, volume 960)


The ability of B lymphocytes to capture external antigens (Ag) and present them as peptide fragments, loaded on Major Histocompatibility complex (MHC) class II molecules, to CD4+ T cells is a crucial part of the adaptive immune response. This allows T-B cooperation, a cellular communication that is required for B cells to develop into germinal centers (GC) and form mature high-affinity antibody producing cells and to further develop B cell memory. MHC class II antigen presentation by B lymphocytes is a multistep process involving (1) Recognition and capture of external Ag by B lymphocytes through their B cell receptor (BCR); (2) Ag processing, which comprises the degradation of Ag in internal compartments within the B cell and loading of the corresponding peptide fragments on MHC class II molecules and (3) Presentation of MHCII-peptide complexes to CD4+ T cells. Here, we describe how to study MHC class II antigen presentation by B lymphocytes at these three major levels.

Key words

B lymphocytes Immune synapse Antigen extraction Processing and presentation MTOC polarization Lysosome secretion 





B cell receptor


Bovine serum albumin


Fetal bovine serum


Germinal center




Immune synapse


Major histocompatibility complex


Microtubule organizing center




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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Institut Curie, Inserm U932ParisFrance

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