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Identifying Source Proteins for MHC Class I-Presented Peptides

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Antigen Processing

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 960))

Abstract

Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells.

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References

  1. Germeau C, Ma W, Schiavetti F, Lurquin C, Henry E, Vigneron N, Brasseur F, Lethé B, De Plaen E, Velu T, Coulie PG (2005) High frequency of anti-tumor T cells in the blood of melanoma patients before and after vaccination with tumor antigens. J Exp Med 201:241–248

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Zarour H, De Smet C, Lehmann F, Marchand M, Lethé B, Romero P, Boon T, Renauld J-C (1996) The majority of autologous cytolytic T-lymphocyte clones derived from peripheral blood lymphocytes of a melanoma patient recognize an antigenic peptide derived from gene Pmel17/gp100. J Invest Dermatol 107:63–67

    Article  CAS  PubMed  Google Scholar 

  3. Kawakami Y, Eliyahu S, Jennings C, Sakaguchi K, Kang X, Southwood S, Robbins PF, Sette A, Appella E, Rosenberg SA (1995) Recognition of multiple epitopes in the human melanoma antigen gp100 by tumor-infiltrating T lymphocytes associated with in vivo tumor regression. J Immunol 154:3961–3968

    CAS  PubMed  Google Scholar 

  4. Espevik T, Nissen-Meyer J (1986) A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J Immunol Methods 95:99–105

    Article  CAS  PubMed  Google Scholar 

  5. Boon T, Van Snick J, Van Pel A, Uyttenhove C, Marchand M (1980) Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. II. T lymphocyte-mediated cytolysis. J Exp Med 152:1184–1193

    Article  CAS  PubMed  Google Scholar 

  6. Hansen MB, Nielsen SE, Berg K (1989) Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 119:203–210

    Article  CAS  PubMed  Google Scholar 

  7. Beyaert R, Vanhaesebroeck B, Suffys P, Van Roy F, Fiers W (1989) Lithium chloride potentiates tumor necrosis factor-mediated cytotoxicity in vitro and in vivo. Proc Natl Acad Sci U S A 86:9494–9498

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Adotevi O, Mollier K, Neuveut C, Cardinaud S, Boulanger E, Mignen B, Fridman WH, Zanetti M, Charneau P, Tartour E, Lemonnier F, Langlade-Demoyen P (2006) Immunogenic HLA-B*0702-restricted epitopes derived from human telomerase reverse transcriptase that elicit antitumor cytotoxic T-cell responses. Clin Cancer Res 12(10):3158–3167. doi:10.1158/1078-0432.CCR-05-2647, 12/10/3158 [pii]

    Article  CAS  PubMed  Google Scholar 

  9. Guéguen M, Patard J-J, Gaugler B, Brasseur F, Renauld J-C, Van Cangh PJ, Boon T, Van den Eynde B (1998) An antigen recognized by autologous CTL on a human bladder carcinoma. J Immunol 160:6188–6194

    PubMed  Google Scholar 

  10. Ma W, Germeau C, Vigneron N, Maernoudt A-S, Morel S, Boon T, Coulie PG, Van den Eynde B (2004) Two new tumor-specific antigenic peptides encoded by gene MAGE-C2 and presented to cytolytic T lymphocytes by HLA-A2. Int J Cancer 109:698–702

    Article  CAS  PubMed  Google Scholar 

  11. Davis LG, Dibner MD, Battey JF (1986) Guanidine isothiocyanate preparation of total RNA. In: Davis LG, Dibner MD, Battey JF (eds) Basic methods in molecular biology. Elsevier, New York, pp 130–135

    Chapter  Google Scholar 

  12. Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell-surface antigens–new tools for genetic analysis. Cell 14:9–20

    Article  CAS  PubMed  Google Scholar 

  13. Yang SY, Morishima Y, Collins NH, Alton T, Pollack MS, Yunis EJ, Dupont B (1984) Comparison of one-dimensional IEF patterns for serologically detectable HLA-A and B allotypes. Immunogenetics 19:217–231

    Article  CAS  PubMed  Google Scholar 

  14. Rebai N, Malissen B (1983) Structural and genetic analyses of HLA class I molecules using monoclonal xenoantibodies. Tissue Antigens 22:107–117

    Article  CAS  PubMed  Google Scholar 

  15. Parham P, Brodsky FM (1981) Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum Immunol 3:277–299

    Article  CAS  PubMed  Google Scholar 

  16. McMichael AJ, Parham P, Rust N, Brodsky F (1980) A monoclonal antibody that recognizes an antigenic determinant shared by HLA-A2 and B17. Hum Immunol 1:121–129

    Article  CAS  PubMed  Google Scholar 

  17. Parham P, Bodmer WF (1978) Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2. Nature 276:397–399

    Article  CAS  PubMed  Google Scholar 

  18. Ellis SA, Taylor C, McMichael A (1982) Recognition of HLA-B27 and related antigen by a monoclonal antibody. Hum Immunol 5(1):49–59. doi:0198-8859(82)90030-1 [pii]

    Article  CAS  PubMed  Google Scholar 

  19. Berger AE, Davis JE, Cresswell P (1982) Monoclonal antibody to HLA-A3. Hybridoma 1(2):87–90

    Article  CAS  PubMed  Google Scholar 

  20. Lehmann F, Marchand M, Hainaut P, Pouillart P, Sastre X, Ikeda H, Boon T, Coulie PG (1995) Differences in the antigens recognized by cytolytic T cells on two successive metastases of a melanoma patient are consistent with immune selection. Eur J Immunol 25:340–347

    Article  CAS  PubMed  Google Scholar 

  21. Van den Eynde BJ, Gaugler B, Probst-Kepper M, Michaux L, Devuyst O, Lorge F, Weynants P, Boon T (1999) A new antigen recognized by cytolytic T lymphocytes on a human kidney tumor results from reverse strand transcription. J Exp Med 190:1793–1799

    Article  PubMed Central  Google Scholar 

  22. De Plaen E, Lurquin C, Lethé B, van der Bruggen P, Brichard V, Renauld J-C, Coulie P, Van Pel A, Boon T (1997) Identification of genes coding for tumor antigens recognized by cytolytic T lymphocytes. Methods 12:125–142

    Article  PubMed  Google Scholar 

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Acknowledgments

We thank J. Klein for editorial assistance. N.V. is a post-doctoral fellow at the Fonds National de la Recherche Scientifique (F.R.S.-F.N.R.S), Belgium and A.M. is supported by a fellowship from the F.R.S.-F.N.R.S (TELEVIE grant No. 7.4592.10).

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Authors and Affiliations

  1. Ludwig Institute for Cancer Research, Brussels Branch and WELBIO and de Duve Institute, Université catholique de Louvain, Brussels, Belgium

    Nathalie Vigneron, Wenbin Ma, Alexandre Michaux & Benoît J. Van den Eynde

Authors
  1. Nathalie Vigneron
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  2. Wenbin Ma
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  3. Alexandre Michaux
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  4. Benoît J. Van den Eynde
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Corresponding author

Correspondence to Benoît J. Van den Eynde .

Editor information

Editors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale, Unité 1013, Université Paris Descartes, Faculté de médecine, Paris, France

    Peter van Endert

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Vigneron, N., Ma, W., Michaux, A., Van den Eynde, B.J. (2013). Identifying Source Proteins for MHC Class I-Presented Peptides. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_16

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  • DOI: https://doi.org/10.1007/978-1-62703-218-6_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-217-9

  • Online ISBN: 978-1-62703-218-6

  • eBook Packages: Springer Protocols

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