Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.)

Part of the Methods in Molecular Biology book series (MIMB, volume 958)


Plant transformation methods are invaluable biotechnological tools to generate specific and targeted genetic variation for performance improvement of crop plants. Genetic information is created by proper modification during gene cloning flanked by proper regulatory sequences and delivered to plants via ­different plant transformation techniques. Due to being a multipurpose plant, cotton has been subjected to different genetic transformation methods to provide the breeders with an opportunity to develop alien traits or improve the endogenous gene performance that are very difficult or impossible to develop through conventional breeding methods. Here we describe the novel physical way of cotton transformation with different genes by using embryogeneic calli as continuous source of explants.


  1. 1.
    Hasnsen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4(6):226–231CrossRefGoogle Scholar
  2. 2.
    Rathore KS, Sunilkumar G, Campbell LM (2010) Cotton: methods in molecular biology, edited by: Kan Wang C, vol 343. Agrobacterium protocols, 2/e, vol 1. Humana Press Inc., Totowa NjGoogle Scholar
  3. 3.
    Wu J, Zhang X, Nie Y, Luo X (2005) High-efficiency transformation of Gossypium hirsutum embryogenic calli mediated by Agrobacterium tumefaciens and regeneration of insect resistant plants. Plant Breed 124:142–146CrossRefGoogle Scholar
  4. 4.
    Asad S, Mukhtar Z, Arshad M (2008) Silicon carbide mediated embryogenic callus transformation of cotton and regeneration of salt tolerant plants. Molecular Biotechnol 40:161–169CrossRefGoogle Scholar
  5. 5.
    Duncan DR (2010) Biotechnology in agriculture and forestry, vol 65. pp 65–77. doi: 10.1007/978-3-642-04796-1-4
  6. 6.
    Petolino JF, Hopkins NL, Kosegi BD, Skokut M (2000) Whisker-mediated transformation of embryogenic callus of maize. Plant Cell Rep 19:781–786CrossRefGoogle Scholar
  7. 7.
    Mizuno K, Takahashi W, Ohyama T, Shimada T, Tanaka O (2004) Improvement of the aluminum borate-whisker medtaed method of DNA delivery into rice callus. Plant Prod SCi 7(1):45–49CrossRefGoogle Scholar
  8. 8.
    Terakawa T, Hisakazu H, Masanori Y (2005) Efficient whiskermediated gene transformation in a combination with supersonic treatment. Breed Sci 55:456–458CrossRefGoogle Scholar
  9. 9.
    Khalafalla M, El-Sheny HA, Rahman SM, Teraishi M, Hasegawa H, Terakawa T, Ishimoto M (2006) Efficient production of transgenic soybean (Glycine max [L] Merrill) plants mediated via whisker-supersonic (WSS) method. Afr J Biotechnol 5(18):1594–1599Google Scholar
  10. 10.
    Matsushita M, Otani M, Wakita M, Tanaki O, Shimida T (1999) Transgenic plant regeneration through silicon carbide mediated transformation of rice (Oryza sativa L.). Breed Sci 49:21–26CrossRefGoogle Scholar
  11. 11.
    Asaad S, Arshad M (2011) Silicon carbide mediated-plant transformation. Properties and application of silicon carbide. ISBN 978-953-307-2012. Intech April, 2011Google Scholar
  12. 12.
    Gaxiola RA, Li J, Undurraga S, Dang LM, Allen GJ, Alper SL, Fink GR (2001) Drought- and salt-tolerant plants results from overexpression of the AVP1 H+  -pump. Proc Natl Acad Sci USA 98:11444–11449PubMedCrossRefGoogle Scholar
  13. 13.
    Hashmi JA, Arshad M, Asad S (2011) Engineering cotton against CLCuD using truncated AC1 sequences. Virus Genes 42(2):286–296PubMedCrossRefGoogle Scholar
  14. 14.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissues cultures. Physiol Plantarum 15:473–479CrossRefGoogle Scholar
  15. 15.
    Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean roots cells. Exp Cell Res 50:150–158CrossRefGoogle Scholar
  16. 16.
    Wilkins TA, Mishra R, Trolinder NL (2004) Agrobacterium mediated transformation regeneration of cotton. Food Agri Environ 2(1):179–187Google Scholar
  17. 17.
    Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: b-glucuronidase as a sensitive and versatile gene fusion in higher plants. EMBO J 6:3901–3907PubMedGoogle Scholar
  18. 18.
    Doyle JJ, Doyle JL (1990) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  19. 19.
    Stewart J, Mc D, Hsu CL (1977) In ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.). Planta 137:113–117CrossRefGoogle Scholar
  20. 20.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Gene Transformation Lab, Agricultural Biotechnology Division (ABD)National Institute for Biotechnology and Genetic Engineering (NIBGE)FaisalabadPakistan

Personalised recommendations