Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.)

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 958)

Abstract

Plant transformation methods are invaluable biotechnological tools to generate specific and targeted genetic variation for performance improvement of crop plants. Genetic information is created by proper modification during gene cloning flanked by proper regulatory sequences and delivered to plants via ­different plant transformation techniques. Due to being a multipurpose plant, cotton has been subjected to different genetic transformation methods to provide the breeders with an opportunity to develop alien traits or improve the endogenous gene performance that are very difficult or impossible to develop through conventional breeding methods. Here we describe the novel physical way of cotton transformation with different genes by using embryogeneic calli as continuous source of explants.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Gene Transformation Lab, Agricultural Biotechnology Division (ABD)National Institute for Biotechnology and Genetic Engineering (NIBGE)FaisalabadPakistan

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