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Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation

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Part of the Methods in Molecular Biology book series (MIMB, volume 958)

Abstract

microRNAs (miRNAs) are an extensive class of newly identified endogenous small regulatory molecules. Many studies show that miRNAs play a critical role in almost all biological and metabolic progresses through targeting protein-coding genes for mRNA cleavage or translation inhibition. Many miRNAs are also identified from cotton using computational and/or experimental approaches, including the next generation deep sequencing technology. However, the function of the majority of miRNAs are unclear. In this chapter, we describe a detailed method for overexpressing miR 156 in cotton using Agrobacterium-mediated genetic transformation. This provides an approach to investigate the function and regulatory mechanism of miRNAs in cotton.

Keywords

Embryogenic Callus Restriction Enzyme BamHI Deep Sequencing Technology Transgenic Cotton Plant Plasmid pBI121 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of BiologyEast Carolina UniversityGreenvilleUSA
  2. 2.Beijing Key Laboratory of Plant Resources Research and Development, Department of Biotechnology, School of ScienceBeijing Technology and Business UniversityBeijingPeople’s Republic of China
  3. 3.Henan Institute of Sciences and TechnologyXinxiangChina

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