Transgenic Cotton

Volume 958 of the series Methods in Molecular Biology pp 161-164


An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants

  • Min WangAffiliated withBeijing Key Laboratory of Plant Resources Research and Development, Department of Biotechnology, School of Science, Beijing Technology and Business University
  • , Qinglian WangAffiliated withHenan Institute of Sciences and Technology
  • , Baohong ZhangAffiliated withDepartment of Biology, East Carolina University Email author 

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Recovery of transgenic cotton plants from tissue culture condition to greenhouse condition is a critical step for improving cotton through genetic engineering. Traditional methods always cause low survival rate of transplanted plants. In 1998, we developed an efficient grafting technique for recovery of transgenic cotton plants, which significantly increased the survival rate of the transplanting regeneration plants. In this chapter, we present a detailed protocol for grafting transgenic cotton plants obtaining somatic embryogenesis.