An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 958)

Abstract

Recovery of transgenic cotton plants from tissue culture condition to greenhouse condition is a critical step for improving cotton through genetic engineering. Traditional methods always cause low survival rate of transplanted plants. In 1998, we developed an efficient grafting technique for recovery of transgenic cotton plants, which significantly increased the survival rate of the transplanting regeneration plants. In this chapter, we present a detailed protocol for grafting transgenic cotton plants obtaining somatic embryogenesis.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Plant Resources Research and Development, Department of Biotechnology, School of ScienceBeijing Technology and Business UniversityBeijingPeople’s Republic of China
  2. 2.Henan Institute of Sciences and TechnologyXinxiangChina
  3. 3.Department of BiologyEast Carolina UniversityGreenvilleUSA

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