Rice Protocols pp 249-255 | Cite as

Inoculation and Virulence Assay for Bacterial Blight and Bacterial Leaf Streak of Rice

  • Bing YangEmail author
  • Adam Bogdanove
Part of the Methods in Molecular Biology book series (MIMB, volume 956)


Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) cause bacterial blight and bacterial leaf streak in rice, respectively. Despite being very closely related, the pathogens colonize different tissues and cause distinct diseases. The diseases are economically important and also serve as model systems for studying plant–bacterial interactions. Here we describe protocols for Xoo and Xoc inoculation and disease scoring methods that are appropriate to their different modes of infection. These methods are routinely used to evaluate pathogen virulence or host responses under controlled environmental conditions.

Key words

Xanthomonas oryzae Inoculation Virulence assay Bacterial blight Bacterial leaf streak Plant–bacterial interactions 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Department of Genetics, Development and Cell BiologyIowa State UniversityAmesUSA
  2. 2.Department of Plant PathologyIowa State UniversityAmesUSA

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