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Rice Protocols pp 243–248Cite as

Analysis of Rice Root Hair Morphology Using Cryo-Scanning Electron Microscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 956))

Abstract

Root hairs are highly polarized long tubular outgrowths from the surface of epidermal trichoblast cells. Root hair development is a simple process that has facilitated for the study of cell fate determination and tissue differentiation in higher plants. Root hair patterning types in dicot and monocot plants are different. Rice is a monocot model plant with type II root hair pattern. The method to examine root hair fine structure and cell shape in rice may help elucidate the mechanism of cell initiation and differentiation in monocot plants. Compared with the critical-point-drying SEM, the Cryo-SEM method has great advantage, as the Cryo-SEM can well maintain the delicate structure of root hairs in their natural situation. Here we provide the methodology developed to investigate several rice mutants with impaired root hair cells using Cryo-SEM.

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References

  1. Gilroy S, Jones DL (2000) Through form to function: root hair development and nutrient uptake. Trends Plant Sci 5:56–60

    Article  PubMed  CAS  Google Scholar 

  2. Gahoonia TS, Care D, Nielsen NE (1997) Root hairs and acquisition of phosphorus by wheat and barley cultivars. Plant Soil 191:181–188

    Article  CAS  Google Scholar 

  3. Bates TR, Lynch JP (2000) The efficiency of Arabidopsis thaliana (Brassicaceae) root hairs in phosphorus acquisition. Am J Bot 87:964–970

    Article  PubMed  CAS  Google Scholar 

  4. Bates TR, Lynch JP (2000) Plant growth and phosphorus accumulation of wild type and two root hair mutants of Arabidopsis thaliana (Brassicaceae). Am J Bot 87:958–963

    Article  PubMed  CAS  Google Scholar 

  5. Zhou J, Jiao FC, Wu ZC, Li YY, Wang XM, He XW, Zhong WQ, Wu P (2008) OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants. Plant Physiol 146:1673–1686

    Article  PubMed  CAS  Google Scholar 

  6. Clowes FAL (2000) Pattern in root meristem development in angiosperms. New Phytol 146:83–94

    Article  Google Scholar 

  7. Dolan L, Costa S (2001) Evolution and genetics of root hair stripes in the root epidermis. J Exp Bot 52:413–417

    Article  PubMed  CAS  Google Scholar 

  8. Kwak SH, Schiefelbein J (2007) The role of the SCRAMBLED receptor-like kinase in patterning the Arabidopsis root epidermis. Dev Biol 302:118–131

    Article  PubMed  CAS  Google Scholar 

  9. Lee MM, Schiefelbein J (1999) WEREWOLF, a MYB-related protein in Arabidopsis, is a position-dependent regulator of epidermal cell patterning. Cell 99:473–483

    Article  PubMed  CAS  Google Scholar 

  10. Schiefelbein JW (2000) Constructing a plant cell. The genetic control of root hair development. Plant Physiol 124:1525–1531

    Article  PubMed  CAS  Google Scholar 

  11. Dolan L, Costa S (2001) Evolution and genetics of root hair stripes in the root epidermis. J Exp Bot 52:413–417

    Article  PubMed  CAS  Google Scholar 

  12. Engvild KC, Rasmussen SK (2004) Root hair mutants of barley. Barley Genet Newsl 34:13–15

    Google Scholar 

  13. Gahoonia TS, Nielsen NE, Joshi PA, Jahoor A (2001) A root hairless barley mutant for elucidating genetic of root hairs and phosphorus uptake. Plant Soil 235:211–219

    Article  CAS  Google Scholar 

  14. Suzuki N, Taketa S, Ichii M (2003) Morphological and physiological characteristics of a root-hairless mutant in rice (Oryza sativa L.). Plant Soil 255:9–17

    Article  CAS  Google Scholar 

  15. Wen TJ, Schnable PS (1994) Analyses of mutants of three genes that are influence root hair development in Zea mays (Gramineae) suggest that root hairs are dispensable. Am J Bot 81:833–842

    Article  Google Scholar 

  16. Kwasniewski M, Szarejko I (2006) Molecular cloning and characterization of beta-expansin gene related to root hair formation in barley. Plant Physiol 141:1149–1158

    Article  PubMed  CAS  Google Scholar 

  17. Kim CM, Park SH, Je BI, Park SH, Park SJ, Piao HL, Eun MY, Dolan L, Han CD (2007) OsCSLD1, a cellulose synthase-like D1 gene, is required for root hair morphogenesis in rice. Plant Physiol 143:1220–1230

    Article  PubMed  CAS  Google Scholar 

  18. Wen TJ, Hochholdinger F, Sauer M, Bruce W, Schnable PS (2005) The roothairless1 gene of maize encodes a homolog of sec3, which is involved in polar exocytosis. Plant Physiol 138:1637–1643

    Article  PubMed  CAS  Google Scholar 

  19. Hochholdinger F, Wen TJ, Zimmermann R, Chimot-Marolle P, da Costa e Silva O, Bruce W, Lamkey KR, Wienand U, Schnable PS (2008) The maize (Zea mays L.) roothairless3 gene encodes a putative GPI-anchored, monocot-specific, COBRA-like protein that significantly affects grain yield. Plant J 54:888–898

    Article  PubMed  CAS  Google Scholar 

  20. Alto 2100 operators handbook. (2005) GATAN

    Google Scholar 

  21. Instruction manual of Model S-3000N scanning electron microscope (2006) HITACHI

    Google Scholar 

Download references

Acknowledgments

This work was supported by the Key Basic Research Special Foundation of China (2005CB120901).

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Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou, China

    Zhiming Yu, Hanmin Chen, Yanli Tong & Ping Wu

Authors
  1. Zhiming Yu
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  2. Hanmin Chen
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  3. Yanli Tong
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  4. Ping Wu
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Corresponding author

Correspondence to Ping Wu .

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Editors and Affiliations

  1. Huck Institutes of Life Sciences, Department of Plant Pathology, Pennsylvania State University, Life Sciences Building 405C, University Park, 16802, Pennsylvania, USA

    Yinong Yang

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Yu, Z., Chen, H., Tong, Y., Wu, P. (2013). Analysis of Rice Root Hair Morphology Using Cryo-Scanning Electron Microscopy. In: Yang, Y. (eds) Rice Protocols. Methods in Molecular Biology, vol 956. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-194-3_17

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  • DOI: https://doi.org/10.1007/978-1-62703-194-3_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-193-6

  • Online ISBN: 978-1-62703-194-3

  • eBook Packages: Springer Protocols

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