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Rice Protocols pp 227-241 | Cite as

Phenotypic, Physiological, and Molecular Evaluation of Rice Chilling Stress Response at the Vegetative Stage

  • Benildo G. de los ReyesEmail author
  • Song Joong Yun
  • Venura Herath
  • Fuyu Xu
  • Myoung Ryoul Park
  • Jeong Il Lee
  • Ki Young Kim
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 956)

Abstract

Rice is a chilling-sensitive plant that is particularly prone to injury during the early stages of seedling development and during flowering. Significant variation exists between subspecies with japonica cultivars generally being less sensitive than most indica cultivars. In most temperate and subtropical countries where rice is grown, crop damage often occurs during the early stages of seedling development due to occasional cold snaps coinciding with the first few weeks after direct seeding in late spring to early summer. Irreversible injuries often result in seedling mortality or if the crop survives a stress episode, plant vigor and resistance to pests and diseases are severely compromised. Recent physiological and molecular studies have shown that oxidative stress is the primary cause of early chilling injuries in rice and the differential responses of indica and japonica cultivars are defined to a large extent by gene expression related to oxidative signaling and defenses. In this chapter, we summarize basic phenotypic, physiological, and molecular procedures that can be adopted for routine evaluation of differential responses between cultivars as well as for functional genomics studies.

Key words

Chilling stress Oxidative stress Reactive oxygen scavenging H2O2 Electrolyte leakage Transcriptome Regulatory cluster 

Notes

Acknowledgement

This work was supported by a grant from USDA-CSREES-NRI, Plant Genome Research (2006-35604-1669), to BGDR and by a grant from Korea Research Foundation (KRF-2006-352-F00002) and BioGreen 21 Program-Rural Development Administration (20080401034024), Republic of Korea, to SJY and MRP.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Benildo G. de los Reyes
    • 1
    Email author
  • Song Joong Yun
    • 2
  • Venura Herath
    • 1
  • Fuyu Xu
    • 1
  • Myoung Ryoul Park
    • 3
    • 4
  • Jeong Il Lee
    • 5
  • Ki Young Kim
    • 6
  1. 1.School of Biology and EcologyUniversity of MaineOronoUSA
  2. 2.Department of Crop Science and Institute of Agricultural Science and TechnologyChonbuk National UniversityJeonjuRepublic of Korea
  3. 3.Department of Crop ScienceChonbuk National UniversityJeonjuRepublic of Korea
  4. 4.Institute of Agricultural Science and TechnologyChonbuk National UniversityJeonjuRepublic of Korea
  5. 5.National Institute of Crop Science, Chuncheon Sub-stationChuncheonRepublic of Korea
  6. 6.Department of Rice and Winter Cereal CropsNational Institute of Crop ScienceIksanRepublic of Korea

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