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Rice Protocols pp 201-208 | Cite as

Analysis of Insect-Induced Volatiles from Rice

  • Nan Zhao
  • Xiaofeng Zhuang
  • Gitika Shrivastava
  • Feng ChenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 956)

Abstract

Plants emit volatile organic compounds (VOCs) from various organs. To understand the biological and ecological roles of plant VOCs as well as their biosynthesis, it is important to determine the quality and quantity of individual plant VOCs. Here we describe three techniques, including solid-phase microextraction, closed-loop stripping system, and an open dynamic system, for sampling volatiles emitted from insect-damaged rice plants. In addition, the protocol for using gas chromatography-mass spectrometry to elucidate the chemical identities of rice volatiles is described.

Key words

Volatile Headspace sampling SPME Closed-loop stripping Rice Insect GC-MS 

Notes

Acknowledgments

The work described was supported by the Experimental Station of Institute of Agriculture, University of Tennessee.

References

  1. 1.
    Pichersky E, Gershenzon J (2002) The formation and function of plant volatiles: perfumes for pollinator attraction and defense. Curr Opin Plant Biol 5:237–243PubMedCrossRefGoogle Scholar
  2. 2.
    Dudareva N, Negre F, Nagegowda DA, Orlova I (2006) Plant volatiles: recent advances and future perspectives. Crit Rev Plant Sci 25:417–440CrossRefGoogle Scholar
  3. 3.
    Yuan JS, Köllner TG, Wiggins G, Grant J, Degenhardt J, Chen F (2008) Molecular and genomic basis of volatile-mediated indirect defense against insects in rice. Plant J 55:491–503PubMedCrossRefGoogle Scholar
  4. 4.
    Ton J, D’Alessandro M, Jourdie V, Jakab G, Karlen D, Held M, Mauch-Mani B, Turlings TCJ (2006) Priming by airborne signals boosts direct and indirect resistance in maize. Plant J 49:16–26PubMedCrossRefGoogle Scholar
  5. 5.
    Dudareva N, Pichersky E, Gershenzon J (2004) Biochemistry of plant volatiles. Plant Physiol 135:1893–1902PubMedCrossRefGoogle Scholar
  6. 6.
    Knudsen JT, Tollsten L, Bergstrom LG (1993) Floral scents: a checklist of volatile compounds isolated by headspace techniques. Phytochemistry 33:253–280CrossRefGoogle Scholar
  7. 7.
    Tholl D, Boland W, Hansel A, Loreto F, Röse US, Schnitzler JP (2006) Practical approaches to plant volatile analysis. Plant J 45:540–560PubMedCrossRefGoogle Scholar
  8. 8.
    Donath J, Boland W (1995) Biosynthesis of acyclic homoterpenes: enzyme selectivity and absolute configuration of the nerolidol precursor. Phytochemistry 39:785–790CrossRefGoogle Scholar
  9. 9.
    Bergstrom J, Bergstriim G (1989) Floral scents of Bartsia alpina (Scrophulariaceae): chemical composition and variation between individual plants. Nordic J Bot 9:363–365CrossRefGoogle Scholar
  10. 10.
    Borg-Karlson A-K (1987) Chemical basis for the relationship between Opkrys orchids and their pollinators. Chem Scr 27:313–325Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Nan Zhao
    • 1
  • Xiaofeng Zhuang
    • 1
  • Gitika Shrivastava
    • 1
  • Feng Chen
    • 1
    Email author
  1. 1.Department of Plant SciencesUniversity of TennesseeKnoxvilleUSA

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