Quantification of Jasmonic and Salicylic Acids in Rice Seedling Leaves
Jasmonic acid (JA) and salicylic acid (SA) are critical signaling components involved in various aspects of plant growth, development, and defense. Their constitutive levels vary from plant to plant and also from tissue to tissue within the same plant. Moreover, their quantitative levels change when plant is exposed to biotic and abiotic stresses. To better understand the JA- and SA-mediated signaling and metabolic pathways, it is important to precisely quantify their levels in plants/tissues/organs. However, their extraction and quantification are not trivial and still technically challenging. An effort has been made in various laboratories to develop a simple and standard procedure that can be utilized for quantification of JA and SA. Here, we present the experimental procedure and our decade of experience on extracting and quantifying them in an absolute manner in leaves of rice seedlings. We must mention that this method has been applied to both monocotyledonous and dicotyledonous plants for absolute quantification of JA and SA. As collaboration is the key towards rapid progress in science and technology, we are always open to sharing our experience in this field with any active research group with an aim to improve the procedure further and eventually to connect the importance of their (JA and SA) quantitative levels with networks of signaling and metabolic pathways in plants.
Key wordsOryza sativa Jasmonic acid Salicylic acid Extraction Quantification
This work was supported by Environment Research and Technology Development Fund (A-0806) of Ministry of Environment, Japan. K.C. was supported at NIES as an Eco-Frontier Fellow (09-Ba086-02). OH appreciates the support of the Korea Research Foundation (2008-313-F00020). GKA and RR greatly appreciate the vision and direction of Prof. V. P. Agrawal (Founder RLABB, Nepal) in their research. GKA appreciates Japan Society for the Promotion of Science (JSPS; ID Number S-10182) for research at NIAS and collaborations therein with ST and RR. RR acknowledges the great support of Professors Yoshihiro Shiraiwa (Chairperson, Faculty of Life and Environmental Sciences, University of Tsukuba) and Seiji Shioda and Dr. Tetsuo Ogawa (Department of Anatomy I, Showa University School of Medicine) in promoting interdisciplinary research and unselfish encouragement.
During the preparation of this manuscript, an advanced method for jasmonates analysis has been reported (22). Moreover, a comprehensive review including recent techniques for jasmonates analysis has also been recently published (23).
- 11.Spoel SH, Koornneef A, Claessens SMC, Korzelius JP, Van Pelt JA, Mueller MJ, Buchala AJ, Metraux JP, Brown R, Kazan K, Van Loon LC, Dong X, Pieterse CMJ (2003) NPR1 modulates cross-talk between salicylate- and jasmonate-dependent defense pathways through a novel function in the cytosol. Plant Cell 15:760–770PubMedCrossRefGoogle Scholar