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Rice Protocols pp 151-184 | Cite as

Rice Proteomic Analysis: Sample Preparation for Protein Identification

  • Ganesh Kumar Agrawal
  • Nam-Soo Jwa
  • Young-Ho Jung
  • Sun Tae Kim
  • Dea-Wook Kim
  • Kyoungwon Cho
  • Junko Shibato
  • Randeep RakwalEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 956)

Abstract

Rice is one of the most important food and cereal crop plants in the world. Rice proteomics began in the 1990s. Since then, considerable progress has been made in establishing protocols from isolation of rice proteins from different tissues, organs, and organelles, to separation of complex proteins and to their identification by mass spectrometry. Since the year 2000, global proteomics studies have been performed during growth and development under numerous biotic and abiotic environmental conditions. Two-dimensional (2-D) gel-based proteomics platform coupled with mass spectrometry has been retained as the workhorse for proteomics of a variety of rice samples. In this chapter, we describe in detail the different protocols used for isolation of rice proteins, their separation, detection, and identification using gel-based proteomics and mass spectrometry approaches.

Key words

Rice Gel-based proteomics One/two-dimensional polyacrylamide gel electrophoresis Mass spectrometry 

Notes

Acknowledgments

GKA and RR appreciate the kind support of Dr. Akihiro Kubo (NIES, Japan) for providing the space to grow and conduct rice stress experiments. GKA appreciates the Japan Society for the Promotion of Science (JSPS; ID Number S-10182) for his stay and research at Plant Genome Research Unit (NIAS, Tsukuba, Japan). This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MEST) (No. 2011-0000139, No. R0602536). This work was also supported by a grant from the BioGreen 21 Program (No. PJ007993), Rural Development Administration, Republic of Korea. K.C. was supported at NIES as an Eco-Frontier Fellow (09-Ba086-02). RR acknowledges the great support of Professors Yoshihiro Shiraiwa (Chairperson, Faculty of Life and Environmental Sciences, University of Tsukuba) and Seiji Shioda and Dr. Tetsuo Ogawa (Department of Anatomy I, Showa University School of Medicine) in promoting interdisciplinary research and unselfish encouragement. For all correspondence, contact Ganesh Kumar Agrawal or Randeep Rakwal at plantproteomics@gmail.com.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Ganesh Kumar Agrawal
    • 1
  • Nam-Soo Jwa
    • 2
  • Young-Ho Jung
    • 2
  • Sun Tae Kim
    • 3
  • Dea-Wook Kim
    • 4
  • Kyoungwon Cho
    • 5
    • 6
  • Junko Shibato
    • 6
    • 7
  • Randeep Rakwal
    • 1
    • 7
    • 8
    Email author
  1. 1.Research Laboratory for Biotechnology and BiochemistryKathmanduNepal
  2. 2.Department of Molecular BiologyCollege of Life Sciences, Sejong UniversitySeoulRepublic of Korea
  3. 3.Department of Plant BiosciencePusan National UniversityBusanRepublic of Korea
  4. 4.National Institute of Crop Science, Rural Development AdministrationSuwonRepublic of Korea
  5. 5.Department of BiotechnologyCollege of Agriculture and Life Sciences, Chonnam National UniversityKwangjuSouth Korea
  6. 6.Environmental Biology DivisionNational Institute for Environmental StudiesTsukubaJapan
  7. 7.Department of Anatomy IShowa University School of MedicineTokyoJapan
  8. 8.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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