Rice Proteomic Analysis: Sample Preparation for Protein Identification
Rice is one of the most important food and cereal crop plants in the world. Rice proteomics began in the 1990s. Since then, considerable progress has been made in establishing protocols from isolation of rice proteins from different tissues, organs, and organelles, to separation of complex proteins and to their identification by mass spectrometry. Since the year 2000, global proteomics studies have been performed during growth and development under numerous biotic and abiotic environmental conditions. Two-dimensional (2-D) gel-based proteomics platform coupled with mass spectrometry has been retained as the workhorse for proteomics of a variety of rice samples. In this chapter, we describe in detail the different protocols used for isolation of rice proteins, their separation, detection, and identification using gel-based proteomics and mass spectrometry approaches.
Key wordsRice Gel-based proteomics One/two-dimensional polyacrylamide gel electrophoresis Mass spectrometry
GKA and RR appreciate the kind support of Dr. Akihiro Kubo (NIES, Japan) for providing the space to grow and conduct rice stress experiments. GKA appreciates the Japan Society for the Promotion of Science (JSPS; ID Number S-10182) for his stay and research at Plant Genome Research Unit (NIAS, Tsukuba, Japan). This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MEST) (No. 2011-0000139, No. R0602536). This work was also supported by a grant from the BioGreen 21 Program (No. PJ007993), Rural Development Administration, Republic of Korea. K.C. was supported at NIES as an Eco-Frontier Fellow (09-Ba086-02). RR acknowledges the great support of Professors Yoshihiro Shiraiwa (Chairperson, Faculty of Life and Environmental Sciences, University of Tsukuba) and Seiji Shioda and Dr. Tetsuo Ogawa (Department of Anatomy I, Showa University School of Medicine) in promoting interdisciplinary research and unselfish encouragement. For all correspondence, contact Ganesh Kumar Agrawal or Randeep Rakwal at email@example.com.
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