Rice Artificial Hybridization for Genetic Analysis

  • Xueyan ShaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 956)


Artificial hybridization has probably been practiced since ancient time; however, the science of genetics did not initiate until Gregor Mendel conducted a series of crosses between different pure lines of garden pea and made careful observations and systematical analyses of their offspring. Artificial hybridization or crossing between carefully chosen parents has been and still is the primary way to transfer genes from different germplasm for self-pollinated rice. Through gene recombination, novel genetic variation is created by different arrangements of genes existing in parental lines. Procedures of artificial hybridization involve the selection of appropriate panicles from representative plants of the female parents, the emasculation of female parents, and the pollination of emasculated panicles with abundant pollens of selected male parents. Of the numerous proposed methods, hot water and vacuum emasculation have proven to be the most robust and reliable ones. A successful and efficient hybridization program also relies on the knowledge of parental lines or germplasm, the reproductive biology and development of rice, the conditions needed to promote flowering and seed development, and the techniques to synchronize flowering of diverse parents.

Key words

Rice Oryza sativa L. Artificial hybridization Crossing Vacuum emasculation Hot water emasculation Pollination 



The author wishes to thank Steve Linscombe, Kris De Pauw, Shane J. Theunissen, and Blake J. Henry for their help with various aspects of this study. Financial support from the Louisiana Rice Research Board is gratefully acknowledged.


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Rice Research Station, Louisiana State University Agricultural CenterCrowleyUSA

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