Making cRNA for Microinjection and Expression of Fluorescently Tagged Proteins for Live-Cell Imaging in Oocytes

  • Mark LevasseurEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 957)


Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.

Key words

Fluorescent proteins In vitro transcription cRNA Oocytes Live cell imaging 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Institute for Cell and Molecular Biosciences, The Medical SchoolNewcastle UniversityNewcastleUK

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