Studying Meiosis-Specific Cohesins in Mouse Embryonic Oocytes
Distinct meiotic cohesin complexes play fundamental roles in various meiosis-specific chromosomal events in spatiotemporally different manners during mammalian meiotic prophase. Immunostaining is one of the essential methods to study meiotic cohesin dynamics. For the study of cohesins in the meiotic prophase of oocytes, ovaries should be taken from the embryos during a very limited period before birth. Here we focus on some technical tips concerning the preparation of oocyte chromosome spreads for immunostaining. Further, we describe a method for chromosome fluorescence in situ hybridization (FISH) against immunostained oocytes.
Key wordsFetal oocyte Embryonic ovary Meiosis Cohesin Prophase Axial element Homolog synapsis Synaptonemal complex Chromosome spread FISH
We thank Dr. Aya Sato for the technical advice on Chromosome FISH with co-immuostaining. This work was supported in part by a JSPS Research Fellowship (to J.K.), a Grant-in-Aid for Scientific Research on Priority Areas, a Grant-in-Aid for Young Scientists (to K.I.), the Global COE Program (Integrative Life Science Based on the Study of Biosignaling Mechanisms), a Grant-in-Aid for Specially Promoted Research (to Y.W.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.