Counting Chromosomes in Intact Eggs
Chromosomal spreads are an established method to assess ploidy in different cell types. However, many traditional chromosome-spreading techniques require dissolution of the cell and can only be used to assess hyperploidy because of potential chromosome loss inherent in the procedure. Here we describe a method to evaluate chromosome numbers in intact eggs so that both hyperploidy and hypoploidy can be accurately detected.
Key wordsChromosome spread Chromosome count Ploidy Monastrol Kinetochores
This work was supported by a grant from the National Institutes of Health (HD 058730) and a Searle Scholar award to M.A.L.