Counting Chromosomes in Intact Eggs

  • Teresa Chiang
  • Michael A. LampsonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 957)


Chromosomal spreads are an established method to assess ploidy in different cell types. However, many traditional chromosome-spreading techniques require dissolution of the cell and can only be used to assess hyperploidy because of potential chromosome loss inherent in the procedure. Here we describe a method to evaluate chromosome numbers in intact eggs so that both hyperploidy and hypoploidy can be accurately detected.

Key words

Chromosome spread Chromosome count Ploidy Monastrol Kinetochores 



This work was supported by a grant from the National Institutes of Health (HD 058730) and a Searle Scholar award to M.A.L.


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan
  2. 2.Department of BiologyUniversity of PennsylvaniaPhiladelphiaUSA

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