Advertisement

Counting Chromosomes in Intact Eggs

  • Teresa Chiang
  • Michael A. LampsonEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 957)

Abstract

Chromosomal spreads are an established method to assess ploidy in different cell types. However, many traditional chromosome-spreading techniques require dissolution of the cell and can only be used to assess hyperploidy because of potential chromosome loss inherent in the procedure. Here we describe a method to evaluate chromosome numbers in intact eggs so that both hyperploidy and hypoploidy can be accurately detected.

Key words

Chromosome spread Chromosome count Ploidy Monastrol Kinetochores 

Notes

Acknowledgements

This work was supported by a grant from the National Institutes of Health (HD 058730) and a Searle Scholar award to M.A.L.

References

  1. 1.
    Duncan FE, Chiang T, Schultz RM, Lampson MA (2009) Evidence that a defective spindle assembly checkpoint is not the primary cause of maternal age-associated aneuploidy in mouse eggs. Biol Reprod 81:768–776PubMedCrossRefGoogle Scholar
  2. 2.
    Mayer TU, Kapoor TM, Haggarty SJ, King RW, Schreiber SL, Mitchison TJ (1999) Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science 286:971–974PubMedCrossRefGoogle Scholar
  3. 3.
    Chiang T, Duncan FE, Schindler K, Schultz RM, Lampson MA (2010) Evidence that weakened centromere cohesion is a leading cause of age-related aneuploidy in oocytes. Curr Biol 20:1522–1528PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Lane SI, Chang HY, Jennings PC, Jones KT (2010) The Aurora kinase inhibitor ZM447439 accelerates first meiosis in mouse oocytes by overriding the spindle assembly checkpoint. Reproduction 140:521–530PubMedCrossRefGoogle Scholar
  5. 5.
    Illingworth C, Pirmadjid N, Serhal P, Howe K, Fitzharris G (2010) MCAK regulates chromosome alignment but is not necessary for preventing aneuploidy in mouse oocyte meiosis I. Development 137:2133–2138PubMedCrossRefGoogle Scholar
  6. 6.
    Chatot CL, Ziomek CA, Bavister BD, Lewis JL, Torres I (1989) An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil 86:679–688PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan
  2. 2.Department of BiologyUniversity of PennsylvaniaPhiladelphiaUSA

Personalised recommendations