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Chromosome Spreads with Centromere Staining in Mouse Oocytes

  • Jean-Philippe Chambon
  • Khaled Hached
  • Katja WassmannEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 957)

Abstract

This chapter describes a technique for performing chromosome spreads from mouse oocytes. It is based on a previously described protocol (Hodges and Hunt, Chromosoma 111: 165–169, 2002), which we have modified. Chromosomes are stained with either Propidium Iodide or Hoechst. This spreading technique allows for simultaneous immunostaining of proteins associated with chromosomes. It is very useful to stain spreads with CREST serum which labels kinetochores, to be able to distinguish bivalents (chromosome pairs), dyads or univalents (paired sister chromatids), and single sister chromatids without ambiguity.

Key words

Chromosome Spreads CREST Meiosis Bivalent Univalent Sister chromatid Cohesion Mouse oocytes 

Notes

Acknowledgments

We thank S. Touati for comments on the manuscript. K.H. was supported through a PhD fellowship by the Ministère de la Recherche and ARC. Research in the group of K.W. is supported by a grant from La Ligue Nationale Contre Le Cancer (Comité d’Ile de France), the UPMC and CNRS.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Jean-Philippe Chambon
    • 1
  • Khaled Hached
    • 2
  • Katja Wassmann
    • 1
    Email author
  1. 1.CNRS UMR7622 Biologie du Développement and UPMC Paris 6ParisFrance
  2. 2.CNRS UMR7622 Biologie du Développement and UPMC Paris 6Bernard, ParisFrance

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