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Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes

  • Kuo-Tai Yang
  • Yi-Nan Lin
  • Shu-Kuei Li
  • Tang K. TangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 957)

Abstract

We previously isolated Aurora-C (Aurkc/Aie1) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment, segregation, kinetochore-microtubule attachment, and cytokinesis in female mouse meiosis. This chapter describes experimental approaches for examining the subcellular localization and function of Aurora-C kinase during female mouse meiosis, presenting detailed methods for introducing exogenous Aurora-C wild-type and kinase-dead mutant mRNAs into mouse oocytes by cytosolic microinjection, and preparing whole-mount meiotic oocytes and chromosome spreads for confocal immunofluorescence microscopy.

Key words

Aurora Oocytes Meiosis Whole-mount Chromosome spreads Microinjection 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Kuo-Tai Yang
    • 1
  • Yi-Nan Lin
    • 1
  • Shu-Kuei Li
    • 1
  • Tang K. Tang
    • 1
    Email author
  1. 1.Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan

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