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Measuring Transport and Accumulation of Radiolabeled Substrates in Oocytes and Embryos

  • Jay M. BaltzEmail author
  • Hannah E. Corbett
  • Samantha Richard
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 957)

Abstract

Radiolabeled compounds that are substrates for transmembrane transporters can be used to study transport and metabolism in mammalian oocytes and preimplantation embryos. Because even very small amounts of radioisotopes can be detected, these techniques are feasible to use with only a few oocytes or embryos, even down to the level of single oocytes or embryos. Here, we describe the methods for determining the transport and accumulation of radiolabeled compounds into oocytes and preimplantation embryos and the determination of the rate of saturable transport via specific transporters in the plasma membrane.

Key words

Embryo Kinetics Oocyte Preimplantation Radioisotope Radiolabeled Transport 

Notes

Acknowledgements

These techniques as carried out in JMB’s laboratory were developed and used in projects that were funded by the Canadian Institutes of Health Research (CIHR) including operating grants and the Program on Oocyte Health. H.E.C. and S.R. were supported by scholarships from the CIHR Institute of Human Development, Child and Youth Health Training Program in Reproduction, Early Development and the Impact on Health (REDIH). We thank Ms. Megan Kooistra for helping validate and edit these protocols.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Jay M. Baltz
    • 1
    Email author
  • Hannah E. Corbett
    • 1
  • Samantha Richard
    • 1
  1. 1.Departments of Obstetrics & Gynecology and Cellular & Molecular Medicine, Ottawa Hospital Research InstituteUniversity of OttawaOttawaCanada

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