Measuring Transport and Accumulation of Radiolabeled Substrates in Oocytes and Embryos
Radiolabeled compounds that are substrates for transmembrane transporters can be used to study transport and metabolism in mammalian oocytes and preimplantation embryos. Because even very small amounts of radioisotopes can be detected, these techniques are feasible to use with only a few oocytes or embryos, even down to the level of single oocytes or embryos. Here, we describe the methods for determining the transport and accumulation of radiolabeled compounds into oocytes and preimplantation embryos and the determination of the rate of saturable transport via specific transporters in the plasma membrane.
Key wordsEmbryo Kinetics Oocyte Preimplantation Radioisotope Radiolabeled Transport
These techniques as carried out in JMB’s laboratory were developed and used in projects that were funded by the Canadian Institutes of Health Research (CIHR) including operating grants and the Program on Oocyte Health. H.E.C. and S.R. were supported by scholarships from the CIHR Institute of Human Development, Child and Youth Health Training Program in Reproduction, Early Development and the Impact on Health (REDIH). We thank Ms. Megan Kooistra for helping validate and edit these protocols.