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Structure–Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography

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Electron Crystallography of Soluble and Membrane Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 955))

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Abstract

Electron crystallography is emerging as an important method in solving protein structures. While it has found extensive applications in the understanding of membrane protein structure and function at a wide range of resolutions, from revealing oligomeric arrangements to atomic models, electron crystallography has also provided invaluable information on the soluble α/β-tubulin which could not be obtained by any other method to date. Examples of critical insights from selected structures of membrane proteins as well as α/β-tubulin are described here, demonstrating the vast potential of electron crystallography that is first beginning to unfold.

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Author information

Authors and Affiliations

  1. School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA

    Tina M. Dreaden & Bridgette A. Barry

  2. School of Biology, Georgia Institute of Technology, Atlanta, GA, USA

    Bharanidharan Devarajan

  3. School of Biology, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA

    Ingeborg Schmidt-Krey

Authors
  1. Tina M. Dreaden
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  2. Bharanidharan Devarajan
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  3. Bridgette A. Barry
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  4. Ingeborg Schmidt-Krey
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Corresponding author

Correspondence to Ingeborg Schmidt-Krey .

Editor information

Editors and Affiliations

  1. School of Biology, Georgia Institute of Technology, Ferst Dr. 310, Atlanta, 30332, Georgia, USA

    Ingeborg Schmidt-Krey

  2. , Dept. Biochemistry & Biophysics, University of California, San Francisco, 16th St. 600, San Francisco, 94158, California, USA

    Yifan Cheng

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Dreaden, T.M., Devarajan, B., Barry, B.A., Schmidt-Krey, I. (2013). Structure–Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography. In: Schmidt-Krey, I., Cheng, Y. (eds) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology, vol 955. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-176-9_27

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  • DOI: https://doi.org/10.1007/978-1-62703-176-9_27

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-175-2

  • Online ISBN: 978-1-62703-176-9

  • eBook Packages: Springer Protocols

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