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Electron Crystallography of Soluble and Membrane Proteins pp 401–426Cite as

Single Particle Electron Microscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 955))

Abstract

Single particle electron microscopy is a versatile technique for the structural analysis of protein complexes in near-native conditions. While tremendous progress has been made during the past few decades in techniques for specimen preparation, imaging, and image analysis, the field is still in development. In the context of this volume on electron crystallography, the following chapter gives practical guidelines on how to begin single particle EM studies, including preparing specimens, selecting imaging conditions, and choosing which of the many approaches to image analysis are appropriate for a specific sample.

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Acknowledgements

We thank Lindsay Baker for a critical reading of this chapter. J.L.R. was supported by a New Investigator award from the Canadian Institutes of Health Research (CIHR). This work was funded by operating grant MOP 81294 from the CIHR.

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  1. Molecular Structure and Function Program, Departments of Biochemistry and Medical Biophysics, The Hospital for Sick Children, The University of Toronto, Toronto, ON, Canada

    Wilson C. Y. Lau & John L. Rubinstein

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  1. Wilson C. Y. Lau
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Correspondence to John L. Rubinstein .

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  1. School of Biology, Georgia Institute of Technology, Ferst Dr. 310, Atlanta, 30332, Georgia, USA

    Ingeborg Schmidt-Krey

  2. , Dept. Biochemistry & Biophysics, University of California, San Francisco, 16th St. 600, San Francisco, 94158, California, USA

    Yifan Cheng

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Lau, W.C.Y., Rubinstein, J.L. (2013). Single Particle Electron Microscopy. In: Schmidt-Krey, I., Cheng, Y. (eds) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology, vol 955. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-176-9_22

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  • DOI: https://doi.org/10.1007/978-1-62703-176-9_22

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