Automation of Data Acquisition in Electron Crystallography

  • Anchi Cheng
Part of the Methods in Molecular Biology book series (MIMB, volume 955)


General considerations for using automation software for acquiring high-resolution images of 2D crystals under low-dose conditions are presented. Protocol modifications specific to this application in Leginon are provided.

Key words

Automation Electron crystallography Electron microscopy Leginon 



This work is supported by National Institutes of Health (NIH) through the National Center for Research Resources (NCRR) P41 program (Grants RR17573 and RR023093). I would like to thank Bridget Carragher for valuable comments and editing of the manuscript.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Cell BiologyThe National Resource for Automated Molecular Microscopy, The Scripps Research InstituteLa JollaUSA

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