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The Caenorhabditis elegans Model of Legionella Infection

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 954))

Abstract

Caenorhabditis elegans can serve as a simple genetic host to study interactions between Legionellaceae and their hosts, and to examine the contribution of specific gene products to virulence and immunity. C. elegans nematodes have several appealing attributes as a host organism; they are inexpensive, have robust genetic analysis tools, have a simple anatomy yet display a wide range of complex behaviors, and, as invertebrates, do not require animal ethics protocols. Use of C. elegans as a host model complements cell-based models, providing additional support and consistency of the experimental data obtained from multiple models. The C. elegans innate immune system functions similarly to that of the alveolar macrophage including the apoptosis [e.g. programmed cell death (PCD)] pathway located within the germline. The digestive tract of C. elegans is a primary interface between the innate immune system and bacterial pathogens. Thus, the C. elegans host model provides an alternative approach to investigate Legionella pneumophila immunopathogenesis.

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Acknowledgments

We thank Mathieu Pinette for his comments and suggestions. This work was supported by a Howard Hughes Medical Institute Early Career Award to C.D.S., and a National Science and Engineering Council Discovery Grant, a Canadian Foundation for Innovation, a Manitoba Medical Service Foundation Award, and a Manitoba Health Research Council Establishment Grant to A.K.C.B.

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Authors and Affiliations

  1. Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada

    Ann Karen C. Brassinga

  2. Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, VA, USA

    Costi D. Sifri

Authors
  1. Ann Karen C. Brassinga
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  2. Costi D. Sifri
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Corresponding author

Correspondence to Ann Karen C. Brassinga .

Editor information

Editors and Affiliations

  1. , Biology of Intracellular Bacteria, Institut Pasteur, Rue du Dr. Roux 28, Paris, 75724, France

    Carmen Buchrieser

  2. Max von Pettenkofer Institute, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, Munich, 80336, Germany

    Hubert Hilbi

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Brassinga, A.K.C., Sifri, C.D. (2013). The Caenorhabditis elegans Model of Legionella Infection. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_27

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  • DOI: https://doi.org/10.1007/978-1-62703-161-5_27

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-160-8

  • Online ISBN: 978-1-62703-161-5

  • eBook Packages: Springer Protocols

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