Protocol

Plant Mineral Nutrients

Volume 953 of the series Methods in Molecular Biology pp 23-45

Date:

Plant Growth and Cultivation

  • Dorina PodarAffiliated withFaculty of Biology and Geology, Department of Experimental Biology, Babes-Bolyai University Email author 

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Abstract

There is a variety of methods used for growing plants indoor for laboratory research. In most cases plant research requires germination and growth of plants. Often, people have adapted plant cultivation protocols to the conditions and materials at hand in their own laboratory and growth facilities. Here I will provide a guide for growing some of the most frequently used plant species for research, i.e., Arabidopsis thaliana, barley (Hordeum vulgare) and rice (Oryza sativa). However, the methods presented can be used for other plant species as well, especially if they are related to the above-mentioned species. The presented methods include growing plants in soil, hydroponics, and in vitro on plates. This guide is intended as a starting point for those who are just beginning to work on any of the above-mentioned plant species. Methods presented are to be taken as suggestive and modification can be made according to the conditions existing in the host laboratory.

Key words

Plant growth Arabidopsis Thlaspi Barley Rice Hydroponics Soil growth In vitro growth Seeds sterilization