Skip to main content

Lipids for Nucleic Acid Delivery: Synthesis and Particle Formation

  • Protocol
  • First Online:
Nanotechnology for Nucleic Acid Delivery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 948))

Abstract

Lipidic vesicles have been extensively studied for their capacity to condensate and deliver nucleic acids to the cells. Many different amphiphilic lipidic structures have been proposed, each of them bringing some advances in nonviral gene transfection. The ionic or neutral nature of the lipids induces tremendous differences in the behavior of the corresponding liposomes, from the complexation of nucleic acid to the delivery to the cell. An efficient delivery in vitro or in vivo also depends closely on the structure of the lipids and very often, efficient liposomes in vitro have been found useless for in vivo administration.

We wish to describe in this chapter the chemical synthesis of two different lipids, one cationic and the other essentially neutral, and the formulation to obtain liposomes and DNA/liposome complexes. The different ways and tricks for the formulation of the two different structures are especially highlighted.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Bangham A, Standish M, Watkins J (1965) Diffusion of univalent ions across the lamellae of swollen phospholipids. J Mol Biol 13:238–252

    Article  CAS  Google Scholar 

  2. Nicolazzi C, Garinot M, Mignet N, Scherman D, Bessodes M (2003) Cationic lipids for transfection. Curr Med Chem 10:1263–1277

    Article  CAS  Google Scholar 

  3. Byk G, Wetzer B, Frederic M, Dubertret C, Pitard B, Jaslin G, Scherman D (2000) Reduction-sensitive lipopolyamines as a novel nonviral gene delivery system for modulated release of DNA with improved transgene expression. J Med Chem 43:4377–4387

    Article  CAS  Google Scholar 

  4. Tranchant I, Thompson B, Nicolazzi C, Mignet N, Scherman D (2004) Physicochemical optimization of plasmid delivery by cationic lipids. J Gene Med. (Suppl. 1):S24–S35

    Google Scholar 

  5. Tranchant I, Mignet N, Crozat E, Chain J, Girard C, Scherman D, Herscovici J (2004) DNA complexing lipopolythiourea. Bioconjug Chem 15:1342–1348

    Article  CAS  Google Scholar 

  6. Leblond J, Mignet N, Largeau C, Seguin J, Scherman D, Herscovici J (2008) Lipopoly­thiourea transfecting agents: lysine thiourea derivatives. Bioconjug Chem 19:306–314

    Article  CAS  Google Scholar 

  7. Breton M, Leblond J, Seguin J, Midoux P, Scherman D, Herscovici J, Pichon C, Mignet N (2010) Comparative gene transfer between cationic and thiourea lipoplexes. J Gene Med 12:45–54

    Article  CAS  Google Scholar 

  8. Thompson B, Mignet N, Hofland H, Lamons D, Seguin J, Nicolazzi C, de la Figuera N, Kuen R, Meng Y, Scherman D, Bessodes M (2005) Neutral post-grafted colloidal particles for gene delivery. Bioconjug Chem 16:608–614

    Article  CAS  Google Scholar 

  9. Leblond J, Mignet N, Largeau C, Spanedda MV, Seguin J, Scherman D, Herscovici J (2007) Lipopolythioureas: a new non-cationic system for gene transfer. Bioconjug Chem 18:484–493

    Article  CAS  Google Scholar 

  10. Mignet N, Scherman D (2010) Anionic pH sensitive lipoplexes. Methods Mol Biol 605:435–444

    Article  CAS  Google Scholar 

  11. Kral T, Leblond J, Hof M, Scherman D, Herscovici J, Mignet N (2010) Lipopolythiourea/DNA interaction: a biophysical study. Biophys Chem 148:68–73

    Article  CAS  Google Scholar 

  12. Bessodes M, Scherman D (2010) Acid-labile liposome/pDNA complexes. Methods Mol Biol 605:405–423

    Article  CAS  Google Scholar 

  13. Turek J, Dubertret C, Jaslin G, Antonakis K, Scherman D, Pitard B (2000) Formulations which increase the size of lipoplexes prevent serum-associated inhibition of transfection. J Gene Med 2:32–40

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Nathalie Mignet .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Bessodes, M., Mignet, N. (2013). Lipids for Nucleic Acid Delivery: Synthesis and Particle Formation. In: Ogris, M., Oupicky, D. (eds) Nanotechnology for Nucleic Acid Delivery. Methods in Molecular Biology, vol 948. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-140-0_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-140-0_6

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-139-4

  • Online ISBN: 978-1-62703-140-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics