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Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement

  • Kerstin Länge
  • Friederike J. Gruhl
  • Michael RappEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 949)

Abstract

Surface acoustic wave (SAW) devices based on horizontally polarized surface shear waves enable direct and label-free detection of proteins in real time. Signal response changes result mainly from mass increase and viscoelasticity changes on the device surface. With an appropriate sensor configuration all types of binding reactions can be detected by determining resonant frequency changes of an oscillator. To create a biosensor, SAW devices have to be coated with a sensing layer binding specifically to the analyte. Intermediate hydrogel layers used within the coating have been proven to be very suitable to easily immobilize capture molecules or ligands corresponding to the analyte. However, aside from mass increase due to analyte binding, the SAW signal response in a subsequent binding experiment strongly depends on the morphology of the sensing layer, as this may lead to different relative changes of viscoelasticity. Bearing these points in mind, we present two basic biosensor coating procedures, one with immobilized capture molecule and a second with immobilized ligand, allowing reliable SAW biosensor signal responses in subsequent binding assays.

Key words

Biosensor Surface acoustic wave SAW SAW resonator Protein coupling Protein detection Surface modification Dextran Polyethylene glycol Carbodiimide chemistry 

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Copyright information

© Springer Science+Business Media,LLC 2013

Authors and Affiliations

  • Kerstin Länge
    • 1
  • Friederike J. Gruhl
    • 1
  • Michael Rapp
    • 1
    Email author
  1. 1.Karlsruhe Institute of Technology (KIT), Institute for Microstructure Technology (IMT)Eggenstein-LeopoldshafenGermany

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