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Charged-Coupled Device (CCD) Detectors for Lab-on-a Chip (LOC) Optical Analysis

  • Avraham RasoolyEmail author
  • Yordan Kostov
  • Hugh A. Bruck
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 949)

Abstract

A critical element of any Lab-on-a-Chip (LOC) is a detector; among the many detection approaches, optical detection is very widely used for biodetection. One challenge for advancing the development of LOC for biodetection has been to enhance the portability and lower the cost for Point-of-Care diagnostics, which has the potential to enhance the quality of healthcare delivery for underserved populations and for global health. We describe a simple and relatively low cost charged-coupled device (CCD)-based detector that can be integrated with a conventional microtiter plate or a portable LOC assay for various optical detection modalities including fluorescence, chemiluminescence, densitometry, and colorimetric assays. In general, the portable battery-operated CCD-based detection system consists of four modules: (1) a cooled CCD digital camera to monitor light emission, (2) a LOC or microtiter plate to perform assays, (3) a light source to illuminate the assay (such as electroluminescence (EL) or light emitting diode (LED)), and (4) a portable computer to acquire and analyze images. The configuration of the fluorescence detector presented here was designed to measure fluorogenic excitation at 490 nm and to monitor emission at 523 nm used for FITC detection.

The LOC used for this detection system was fabricated with laminated object manufacturing (LOM) technology, and was designed to detection activity of botulinum neurotoxin serotype A (BoNT-A) using a fluorogenic peptide substrate (SNAP-25) for botulinum neurotoxin serotype A (BoNT-A) labeled with FITC. The limit of detection (LOD) for the CCD detector is 0.5 nM (25 ng/ml). The portable system is small and is powered by a 12 V source. The modular detector was designed with easily interchangeable LEDs, ELs, filters, lenses, and LOC, and can be used and adapted for a wide variety of densitometry, florescence and colorimetric assays.

Key words

LED Electroluminescence (EL) CCD Fluorescence Laminated object manufacturing (LOM) Fluorometer Botulinum neurotoxin 

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Copyright information

© Springer Science+Business Media,LLC 2013

Authors and Affiliations

  • Avraham Rasooly
    • 1
    • 2
    Email author
  • Yordan Kostov
    • 3
    • 4
  • Hugh A. Bruck
    • 5
  1. 1.Division of Biology, Office of Science and EngineeringFDA Center for Devices and Radiological Health (CDRH)Silver SpringUSA
  2. 2.National Cancer InstituteRockvilleUSA
  3. 3.Steven Sun Division of Biology Office of Science and EngineeringFDA Center for Devices and Radiological Health (CDRH)Silver SpringUSA
  4. 4.University of Maryland Baltimore CountyBaltimore CountyUSA
  5. 5.Department of Mechanical EngineeringUniversity of MarylandCollege ParkUSA

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