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Applications of Microfluidics for Molecular Diagnostics

  • Harikrishnan Jayamohan
  • Himanshu J. Sant
  • Bruce K. GaleEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 949)

Abstract

Diagnostic assays implemented in microfluidic devices have developed rapidly over the past decade and are expected to become commonplace in the next few years. Hundreds of microfluidics-based approaches towards clinical diagnostics and pathogen detection have been reported with a general theme of rapid and customizable assays that are potentially cost-effective. This chapter reviews microfluidics in molecular diagnostics based on application areas with a concise review of microfluidics in general. Basic principles of microfabrication are briefly reviewed and the transition to polymer fabricated devices is discussed. Most current microfluidic diagnostic devices are designed to target a single disease, such as a given cancer or a variety of pathogens, and there will likely be a large market for these focused devices; however, the future of molecular diagnostics lies in highly multiplexed microfluidic devices that can screen for potentially hundreds of diseases simultaneously.

Key words

Microfluidics Micro-total-analysis-systems Lab-on-a-chip Point-of-care devices Sample preparation MEMS Rapid prototyping Biomarker detection Personalized medicine Global health care 

Notes

Acknowledgments

The authors thank Keng-Min Lin for the schematic diagram in Fig. 1. The authors would like to thank the Nano Institute of Utah for funding this work through a nanotechnology training fellowship.

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Copyright information

© Springer Science+Business Media,LLC 2013

Authors and Affiliations

  • Harikrishnan Jayamohan
    • 1
  • Himanshu J. Sant
    • 1
  • Bruce K. Gale
    • 1
    Email author
  1. 1.Department of Mechanical Engineering, State of Utah Center of Excellence for Biomedical MicrofluidicsUniversity of UtahSalt Lake CityUSA

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