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Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET

  • Monpichar Srisa-Art
  • Sanjiv SharmaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 949)

Abstract

Droplet microfluidic systems provide a controlled environment in which to perform rapid mixing, isolation of picoliter size fluid volumes and rapid variation of reaction conditions. Indeed, the ability to controllably form droplets with variable reagent composition at high speed lies at the heart of performance improvements when compared to conventional microfluidic devices operating under laminar flow conditions. Furthermore, it is important to realize that segmented-flow systems can generate droplets at rates in excess of 1 kHz. In theory, this means that millions of individual reactions or assays can be processed in very short times. In addition, since mixing is rapid and reagent transport occurs with no dispersion, microdroplet reactors are superior environments in which to study biological reactions, especially rapid kinetic reactions, when compared to diffusion-limited continuous-flow formats. Accordingly, droplet microfluidics is a promising technology to perform reactions and assays in a high-throughput manner, in which a hugely productive and efficient system for screening a desired component from thousands of samples is necessary.

Key words

Droplet microfluidics Microdroplet reactors Binding assays Binding kinetics 

Notes

Acknowledgements

M. Srisa-Art would like to thank Prof Andrew J. deMello and Dr Joshua B. Edel for their supervision, advice, and encouragement and also acknowledge the Royal Thai Government for provision of a research scholarship. This work was supported by the EPSRC and the RCUK Basic Technology Programme.

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Copyright information

© Springer Science+Business Media,LLC 2013

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  2. 2.Institute of Biomedical Engineering & Department of ChemistryImperial CollegeLondonUK

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