Microfluidic Interface Technology Based on Stereolithography for Glass-Based Lab-on-a-Chips

  • Song-I Han
  • Ki-Ho HanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 949)


As lab-on-a-chips are developed for on-chip integrated microfluidic systems with multiple functions, the development of microfluidic interface (MFI) technology to enable integration of complex microfluidic systems becomes increasingly important and faces many technical difficulties. Such difficulties include the need for more complex structures, the possibility of biological or chemical cross-contamination between functional compartments, and the possible need for individual compartments fabricated from different substrate materials. This chapter introduces MFI technology, based on rapid stereolithography, for a glass-based miniaturized genetic sample preparation system, as an example of a complex lab-on-a-chip that could include functional elements such as; solid-phase DNA extraction, polymerase chain reaction, and capillary electrophoresis. To enable the integration of a complex lab-on-a-chip system in a single chip, MFI technology based on stereolithography provides a simple method for realizing complex arrangements of one-step plug-in microfluidic interconnects, integrated microvalves for microfluidic control, and optical windows for on-chip optical processes.

Key words

Microfluidic interfaces Stereolithography Lab-on-a-chip Microfluidic interconnects Microvalves Optical windows Genetic sample preparation system 


  1. 1.
    Terry SC, Jerman JH, Angell JB (1979) A gas chromatographic air analyzer fabricated on a silicon wafer. IEEE T Electron Dev ED-26:1880–1886CrossRefGoogle Scholar
  2. 2.
    Bakajin O, Duke TAJ, Tegenfeldt J, Chou C-F, Chan SS, Austin RH, Cox EC (2001) Separation of 100 kilobase DNA molecules in 10 seconds. Anal Chem 73:6053–6056CrossRefGoogle Scholar
  3. 3.
    Huang Y, Joo S, Duhon M, Heller M, Wallace B, Xu X (2002) Dielectrophoretic cell separation and gene expression profiling on microelectronic chip arrays. Anal Chem 74:3362–3371CrossRefGoogle Scholar
  4. 4.
    Fu AY, Spence C, Scherer A, Arnold FH, Quake SR (1999) A microfabricated fluorescence-activated cell sorter. Nat Biotechnol 11:1109–1111Google Scholar
  5. 5.
    Chang KS, Tanaka S, Chang CL, Esashi M, G. Benjamin Hocker (2003). The Institute of Electrical and Electronics Engineers, Inc. (IEEE). Combustor-integrated micro-fuel processor with suspended membrane structure. In: Tech dig 12th int. conf. solid-state sensors and actuators workshop (Transducers’03), Boston, USA, 2003, pp 635–638Google Scholar
  6. 6.
    Han K-H, Frazier AB (2006) Paramagnetic capture mode magnetophoretic microseparator for high efficiency blood cell separations. Lab Chip 6:265–273CrossRefGoogle Scholar
  7. 7.
    Man PF, Jones DK, Mastrangelo CH, Kazuo Sato and Shuichi Shoji (1997). The Institute of Electrical and Electronics Engineers, Inc. (IEEE). Microfuidic plastic capillaries on silicon substrates: a new inexpensive technology for bioanalysis chips. In: International workshop on micro electromechanical systems (MEMS 97), Nagoya, Japan, pp 311–316Google Scholar
  8. 8.
    Grover WH, Skelley AM, Lui CN, Lagally ET, Mathies RA (2003) Monolithic membrane valves and diaphragm pumps for practical large-scale integration into glass microfluidic devices. Sensor Actuat B 89:315–323CrossRefGoogle Scholar
  9. 9.
    Han A, Graff M, Mohanty SK, Edwards TL, Han K-H, Frazier AB (2003) Multi-layer plastic/glass microfluidic systems containing electrical and mechanical functionality. Lab Chip 3:150157Google Scholar
  10. 10.
    Fredrickson K, Fan ZH (2004) Macro-to-micro interfaces for microfluidic devices. Lab Chip 4:526–533CrossRefGoogle Scholar
  11. 11.
    Stachowiak TB, Rohr T, Hilder EF, Peterson DS, Yi M, Svec F, Fréchet JMJ (2003) Fabrication of porous polymer monoliths covalently attached to the walls of channels in plastic microdevices. Electrophoresis 24:3689–3693Google Scholar
  12. 12.
    Pattekar V, Kothare MV (2003) Novel microfluidic interconnectors for high temperature and pressure applications. J Micromech Microeng 13:337–345Google Scholar
  13. 13.
    Chen H, Acharya D, Gajraj A, Meiners J-C (2003) Robust interconnects and packaging for microfluidic elastomer chips. Anal Chem 75:5287–5291CrossRefGoogle Scholar
  14. 14.
    Gray BL, Jaeggi D, Mourlas NJ, van Drieenhuizen BP, Williams KR, Maluf NI, Kovacs GTA (1999) Novel interconnection technologies for integrated microfluidic systems. Sensor Actuat A 77:57–65CrossRefGoogle Scholar
  15. 15.
    Nittis V, Fortt R, Legge CH, de Mello AJ (2001) A high-pressure interconnect for chemical microsystem applications. Lab Chip 1:148–152CrossRefGoogle Scholar
  16. 16.
    Verlee D, Alcock A, Clark G, Huang TM, Kantor S, Nemcek T, Norlie J, Pan J, Walsworth F, Wong ST (1996) Fluid circuit technology: integrated interconnect technology for miniature fluidic devices. Tech Dig Solid State Sensor Actuat (Hilton Head, USA 1996:9–14Google Scholar
  17. 17.
    Yao TJ, Lee S, Fang W, Tai Y-C, Hiroki Kuwano and Isao Shimoyama (2000). The Institute of Electrical and Electronics Engineers, Inc. (IEEE). A micromachined rubber O-ring microfluidic coupler. In: Proc IEEE micro electro mechanical systems conference (MEMS 2000), Miyazaki, Japan, 2000, pp 624–627Google Scholar
  18. 18.
    Meng E, Wu S, Tai Y-C, A. van den Berg, W. Olthuis and P. Bergveld (2000). Kluwer Academic Publishers. Micromachined fluidic couplers. In: Proceedings of the micro total analysis systems symposium (μTAS), Enschede, Netherlands, 2000, pp 41–44Google Scholar
  19. 19.
    González C, Collins SD, Smith RL (1998) Fluidic interconnects for modular assembly of chemical microsystems. Sensor Actuat B 49:40–45CrossRefGoogle Scholar
  20. 20.
    Gray BL, Collins SD, Smith RL (2004) Interlocking mechanical and fluidic interconnections for microfluidic circuit boards. Sensor Actuat A 112:18–24CrossRefGoogle Scholar
  21. 21.
    Puntambekar CA, Ahn CH (2002) Self-aligning microfluidic interconnects for glass- and plastic-based microfluidic systems. J Micromech Microeng 12:35–40Google Scholar
  22. 22.
    Yang Z, Maeda R (2003) Socket with built-in valves for the interconnection of microfluidic chips to macro constituents. J Chromatogr A 1013:29CrossRefGoogle Scholar
  23. 23.
    Kovacs GTA (1998) Micromachined transducers sourcebook. McGraw-Hill Co., BostonGoogle Scholar
  24. 24.
    Hosokawa K, Maeda R (2000) A pneumatically-actuated three-way microvalve fabricated with polydimethylsiloxane using the membrane transfer technique. J Micromech Microeng 10:415–420CrossRefGoogle Scholar
  25. 25.
    Wang Y-C, Choi MH, Han J (2004) Two-dimensional protein separation with advanced sample and buffer isolation using microfluidic valves. Anal Chem 76:4426–4431CrossRefGoogle Scholar
  26. 26.
    Lee S, Jeong W, Beebe DJ (2003) Microfluidic valve with cored glass microneedle for microinjection. Lab Chip 3:164–167CrossRefGoogle Scholar
  27. 27.
    Ren X, Bachman M, Sims C, Li GP, Allbritton N (2001) Electroosmotic properties of microfluidic channels composed of poly (dimethysiloxane). J Chromatogr B 762:117–125CrossRefGoogle Scholar
  28. 28.
    Hu S, Ren X, Bachman M, Sims CE, Li GP, Allbritton N (2002) Surface modification of poly(dimethylsiloxane) microfluidic devices by ultraviolet polymer grafting. Anal Chem 74:4117–4123CrossRefGoogle Scholar
  29. 29.
    Wolfe KA, Breadmore MC, Ferrance JP, Power ME, Conroy JF, Norris PM, Landers JP (1997) Toward a microchip-based solid-phase extraction method for isolation of nucleic acids. Electrophoresis 23:727–733CrossRefGoogle Scholar
  30. 30.
    Harrison DJ, Fluri K, Seiler K, Fan Z, Effenhauser CS, Manz A (1993) Micro­machining a miniaturized capillary electrophoresis based chemical analysis system on a chip. Science 261:895–897CrossRefGoogle Scholar
  31. 31.
    Giordano BC, Jin L, Couch AJ, Ferrance JP, Landers JP (2004) Microchip laser-induced fluorescence detection of proteins at submicrogram per milliliter levels mediated by dynamic labeling under pseudonative conditions. Anal Chem 76:4705–4714CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media,LLC 2013

Authors and Affiliations

  1. 1.School of Nano EngineeringInje UniversityGimhaeRepublic of Korea

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