The Development of T Lymphocytes in Fetal Thymus Organ Culture

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 946)

Abstract

Fetal thymus organ culture (FTOC) is a unique and powerful culture system that allows intrathymic T-lymphocyte development in vitro. T-cell development in FTOC well represents fetal thymocyte development in vivo. Here we describe the basic method for FTOC as well as several related techniques, including reconstitution of thymus lobes with T-lymphoid progenitor cells, high-oxygen submersion culture, reaggregation thymus organ culture, retrovirus-mediated gene transfer to developing thymocytes in FTOC, and coculture of progenitor cells with OP9-DL1 cells.

Key words

T lymphocyte Fetal thymus Organ culture Retrovirus Flow cytometry 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Takeshi Nitta
    • 1
  • Izumi Ohigashi
    • 1
  • Yousuke Takahama
    • 2
  1. 1.Division of Experimental Immunology, Institute for Genome ResearchUniversity of TokushimaTokushimaJapan
  2. 2.Division of Experimental Immunology, Institute for Genome ResearchThe University of TokushimaTokushimaJapan

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