Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 946)

Abstract

Many human embryonic stem (hES) and induced pluripotent stem (hiPS) cell differentiation protocols begin with the formation of three-dimensional aggregates of cells called embryoid bodies (EBs). Traditional EB formation methods result in a heterogeneous population of EB sizes and shapes, which then undergo heterogeneous differentiation efficiencies. AggreWellTM400 and AggreWellTM800 use the spin-EB method to force the aggregation of a defined number of cells, thereby controlling EB size and generating a population of uniform EBs. Moreover, the dense array of microwells on the bottom surface of AggreWellTM400 provide for the rapid and simple production of thousands of EBs at a time.

Key words

Human embryonic stem cell Embryoid bodies Induced pluripotent stem cells 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.STEMCELL Technologies IncVancouverCanada

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