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Viral Transformation of Epithelial Cells

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Epithelial Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 945))

Abstract

Approximately 18% of human cancers have a viral etiology and the majority of these involve transformation of epithelial cells. Viral proteins transform epithelia by inducing alterations in the normal cell growth and differentiation pathways through the targeting of host proteins. Among the DNA viruses responsible for causing carcinomas are the human papillomaviruses as well as several members of the herpes and polyomavirus families. A number of techniques have been developed to study the mechanisms by which viruses immortalize epithelial cells and alter differentiation properties. These methods include the generation of immortalized lines by transfection or infection as well as the use of organotypic raft cultures, suspension in methylcellulose, and treatment with high calcium levels to examine how differentiation is altered.

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Authors and Affiliations

  1. Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA

    Jennifer A. Regan & Laimonis A. Laimins

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  1. Jennifer A. Regan
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  2. Laimonis A. Laimins
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Correspondence to Laimonis A. Laimins .

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Editors and Affiliations

  1. Cystic Fibrosis/Pulmonary Research Ctr., University of North Carolina, Thurston Bowles Building 4011, Chapel Hill, 27599, North Carolina, USA

    Scott H. Randell

  2. Cystic Fibrosis/Pulmonary Research Ctr., The University of North Carolina, Thurston-Bowles Building 4011, Chapel Hill, 27599, North Carolina, USA

    M. Leslie Fulcher

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Regan, J.A., Laimins, L.A. (2012). Viral Transformation of Epithelial Cells. In: Randell, S., Fulcher, M. (eds) Epithelial Cell Culture Protocols. Methods in Molecular Biology, vol 945. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-125-7_26

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  • DOI: https://doi.org/10.1007/978-1-62703-125-7_26

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  • Publisher Name: Humana Press, Totowa, NJ

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