Phytoplasma pp 109-113 | Cite as

Dienes’ Staining and Light Microscopy for Phytoplasma Visualization

  • Rita MusettiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 938)


The chapter describes the main light microscopy techniques used for the diagnosis of phytoplasmas. Because the described techniques are generally only effective in cases where the concentration of the pathogen inside the host phloem tissue is high, they are useful as preliminary methods to assess the presence of phytoplasmas and analyze the distribution in symptomatic plants.

Key words

Detection Dienes’ stain Light microscopy Phytoplasma 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Department of Agricultural and Environmental SciencesUniversity of UdineUdineItaly

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