Phytoplasma pp 405-416 | Cite as

Mapping the Phytoplasma Chromosome

  • Sylvie Malembic-MaherEmail author
  • Patricia Carle
Part of the Methods in Molecular Biology book series (MIMB, volume 938)


Physical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation of phytoplasma chromosomes from infected plants, single- and double-digestion of chromosomes with rare-cutting restriction enzymes, separation of large DNA fragments by pulsed-field gel electrophoresis, and hybridization with various genetic markers. Materials and methods needed for each step are described and the technique is illustrated using the flavescence dorée phytoplasma genome map as an example.

Key words

Flavescence dorée phytoplasma Genetic map Mollicutes Physical map Pulsed-field gel electrophoresis Restriction endonucleases Southern blot 



This research was funded by the French National Institute of Agronomical Research (INRA) and by the Interprofessional Council of Bordeaux Wine (CIVB). We thank our colleagues Pascal Salar and Géraldine Gourgues for elaborating the protocols for phytoplasma chromosome isolation and for PFGE.


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.UMR1332 Fruit Biology and PathologyINRA, University of BordeauxVillenave d’OrnonFrance

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