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Phytoplasma pp 371-379 | Cite as

Phytoplasma Plasmid DNA Extraction

  • Mark T. AndersenEmail author
  • Lia W. Liefting
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 938)

Abstract

Phytoplasma plasmids have generally been detected from DNA extracted from plants and insects using methods designed for the purification of total phytoplasma DNA. Methods include extraction from tissues that are high in phytoplasma titre, such as the phloem of plants, with the use of CsCl–bisbenzimide gradients that exploit the low G+C content of phytoplasma DNA. Many of the methods employed for phytoplasma purification have been described elsewhere in this book. Here we describe in detail two methods that are specifically aimed at isolating plasmid DNA.

Key words

Alkaline lysis CsCl gradients Plasmid 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Plant & Food ResearchAucklandNew Zealand
  2. 2.Plant Health and Environment LaboratoryMinistry for Primary IndustriesAucklandNew Zealand

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