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Phytoplasma pp 291-300 | Cite as

In-Field Diagnostics Using Loop-Mediated Isothermal Amplification

  • Jenny TomlinsonEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 938)

Abstract

Loop-mediated isothermal amplification (LAMP) is a method for amplification and detection of target organisms which, unlike polymerase chain reaction, does not require thermal cycling. LAMP assays can be developed in the laboratory for subsequent deployment in the field, where the simplicity of isothermal amplification makes LAMP a suitable method for rapid detection of phytoplasmas with levels of sensitivity and specificity approaching those of more complex and time-consuming laboratory methods.

Key words

Detection On-site testing Loop-mediated isothermal amplification Polymerase chain reaction Primer design 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.The Food and Environment Research AgencyYorkUK

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