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Phytoplasma pp 283-289 | Cite as

Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions

  • Paolo Margaria
  • Sabrina PalmanoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 938)

Abstract

Phytoplasmas are routinely detected by nucleic acid-based techniques. These approaches rely on enriched phytoplasma DNA extracts of good quality, following labor intensive and time-consuming purification protocols. Here we describe a very rapid, specific, sensitive, and reliable method for flavescence dorée phytoplasma detection, based on real-time Taqman® reverse transcription-PCR of the 16S rRNA. The protocol is particularly useful for large-scale screening of vineyards and nurseries, pathogen surveys, and field epidemiological studies.

Key words

Crude sap Grapevine Real-time TaqMan® reverse transcription-PCR Ribosomal RNA 

Notes

Acknowledgements

P. M. was supported by the grant “Studi sui fattori che favoriscono le epidemie di flavescenza dorata in Piemonte e loro superamento,” sub-project C, from Regione Piemonte, Italy (Report number 12851).

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Istituto di Virologia Vegetale, CNRTorinoItaly

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