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Phytoplasma pp 245-252 | Cite as

Real-Time PCR for Universal Phytoplasma Detection and Quantification

  • Nynne Meyn Christensen
  • Henriette Nyskjold
  • Mogens NicolaisenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 938)

Abstract

Currently, the most efficient detection and precise quantification of phytoplasmas is by real-time PCR. Compared to nested PCR, this method is less sensitive to contamination and is less work intensive. Therefore, a universal real-time PCR method will be valuable in screening programs and in other setups in which large numbers of samples are tested.

Key words

Phytoplasma Quantification Real-time PCR Universal detection 

References

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Nynne Meyn Christensen
    • 1
  • Henriette Nyskjold
    • 2
  • Mogens Nicolaisen
    • 2
    Email author
  1. 1.Center for Advanced Bioimaging (CAB)University of CopenhagenCopenhagenDenmark
  2. 2.Department of AgroecologyAarhus UniversitySlagelseDenmark

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