Advertisement

Phytoplasma pp 15-32 | Cite as

Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus

  • Jennifer HodgettsEmail author
  • David Crossley
  • Matt Dickinson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 938)

Abstract

Phytoplasma collections are a vital resource for researchers and diagnosticians studying phytoplasma diseases. They provide material as a point of reference and a research tool to increase our understanding of phytoplasmas and the diseases they cause. This chapter describes the techniques required to create and maintain collections of phytoplasma-infected Catharanthus roseus (Madagascar periwinkle).

Key words

Catharanthus roseus Cuttings Glasshouse maintenance Grafting technique Phytoplasma 

Notes

Acknowledgements

We would like to acknowledge Professor Phil Jones and the horticultural staff at the University of Nottingham and the Food and Environment Research Agency for training and guidance provided on the creation and maintenance of phytoplasma collections. We would also like to thank all individuals and organizations that have provided us with phytoplasma-infected plant samples.

References

  1. 1.
    Seemuller E et al (1998) Current status of molecular classification of the phytoplasmas. J Plant Pathol 80:3–26Google Scholar
  2. 2.
    Choi YH et al (2004) Metabolic discrimination of Catharanthus roseus leaves infected by phytoplasma using 1H-NMR spectroscopy and multivariate data analysis. Plant Physiol 135:2398–2410PubMedCrossRefGoogle Scholar
  3. 3.
    Favali MA et al (2004) Catharanthus roseus L. plants and explants infected with phytoplasmas: alkaloid production and structural observations. Protoplasma 223:45–51PubMedCrossRefGoogle Scholar
  4. 4.
    Pati PK, Kaur J, Singh P (2011) A liquid culture system for shoot proliferation and analysis of pharmaceutically active constituents of Catharanthus roseus (L.) G. Don. Plant Cell Tissue Organ Cult 105:299–307CrossRefGoogle Scholar
  5. 5.
    Aslam J et al (2010) Catharanthus roseus (L.) G. Don. an important drug: it’s applications and production. Pharm Glob 1:1–16Google Scholar
  6. 6.
    Abdul JA et al (2007) Antioxidant potentials and ajmalicine accumulation in Catharanthus roseus after treatment with gibberellic acid. Colloids Surf 60:195–200CrossRefGoogle Scholar
  7. 7.
    Gupta S et al (2007) Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus. J Genet 86:259–268PubMedCrossRefGoogle Scholar
  8. 8.
    Lee I-M et al (2004) ‘Candidatus Phytoplasma asteris’, a novel phytoplasma taxon associated with aster yellows and related diseases. Int J Syst Evol Microbiol 54:1037–1048PubMedCrossRefGoogle Scholar
  9. 9.
    Kakizawa S (2008) Genome sequencing and analysis of cell surface membrane proteins of phytoplasmas. J Gen Plant Pathol 74:457–548CrossRefGoogle Scholar
  10. 10.
    Berges R, Rott M, Seemüller E (2000) Range of phytoplasma concentrations in various plant hosts as determined by competitive polymerase chain reaction. Phytopathology 90:1145–1152PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Jennifer Hodgetts
    • 1
    Email author
  • David Crossley
    • 1
  • Matt Dickinson
    • 2
  1. 1.The Food and Environment Research AgencyYorkUK
  2. 2.School of BiosciencesUniversity of NottinghamNottinghamUK

Personalised recommendations