Phytoplasma pp 15-32 | Cite as

Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus

  • Jennifer HodgettsEmail author
  • David Crossley
  • Matt Dickinson
Part of the Methods in Molecular Biology book series (MIMB, volume 938)


Phytoplasma collections are a vital resource for researchers and diagnosticians studying phytoplasma diseases. They provide material as a point of reference and a research tool to increase our understanding of phytoplasmas and the diseases they cause. This chapter describes the techniques required to create and maintain collections of phytoplasma-infected Catharanthus roseus (Madagascar periwinkle).

Key words

Catharanthus roseus Cuttings Glasshouse maintenance Grafting technique Phytoplasma 



We would like to acknowledge Professor Phil Jones and the horticultural staff at the University of Nottingham and the Food and Environment Research Agency for training and guidance provided on the creation and maintenance of phytoplasma collections. We would also like to thank all individuals and organizations that have provided us with phytoplasma-infected plant samples.


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Jennifer Hodgetts
    • 1
    Email author
  • David Crossley
    • 1
  • Matt Dickinson
    • 2
  1. 1.The Food and Environment Research AgencyYorkUK
  2. 2.School of BiosciencesUniversity of NottinghamNottinghamUK

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