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Automated DNA Extraction for Large Numbers of Plant Samples

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Phytoplasma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 938))

Abstract

The method described here is a rapid, total DNA extraction procedure applicable to a large number of plant samples requiring pathogen detection. The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads. DNA is purified in an automated process in which the magnetic beads are transferred through a series of washing buffers. The eluted DNA is suitable for efficient amplification in PCR reactions.

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References

  1. Boben J, Mehle N, Ravnikar M (2007) Optimization of extraction procedure can improve phytoplasma diagnostics. Bull Insectol 60:249–250

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  2. Nikolić P et al (2010) A panel of real-time PCR assays for specific detection of three phytoplasmas from the apple proliferation group. Mol Cell Probes 24:303–309

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  3. Pirc M et al (2009) Improved fireblight diagnostics using quantitative real-time PCR detection of Erwinia amylovora chromosomal DNA. Plant Pathol 58:872–881

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Correspondence to Nataša Mehle .

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Mehle, N., Nikolić, P., Rupar, M., Boben, J., Ravnikar, M., Dermastia, M. (2013). Automated DNA Extraction for Large Numbers of Plant Samples. In: Dickinson, M., Hodgetts, J. (eds) Phytoplasma. Methods in Molecular Biology, vol 938. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-089-2_12

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  • DOI: https://doi.org/10.1007/978-1-62703-089-2_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-088-5

  • Online ISBN: 978-1-62703-089-2

  • eBook Packages: Springer Protocols

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