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Phytoplasma pp 139-145 | Cite as

Automated DNA Extraction for Large Numbers of Plant Samples

  • Nataša MehleEmail author
  • Petra Nikolić
  • Matevž Rupar
  • Jana Boben
  • Maja Ravnikar
  • Marina Dermastia
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 938)

Abstract

The method described here is a rapid, total DNA extraction procedure applicable to a large number of plant samples requiring pathogen detection. The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads. DNA is purified in an automated process in which the magnetic beads are transferred through a series of washing buffers. The eluted DNA is suitable for efficient amplification in PCR reactions.

Key words

Phytoplasma Homogenization DNA extraction Automation Magnetic processor 

References

  1. 1.
    Boben J, Mehle N, Ravnikar M (2007) Optimization of extraction procedure can improve phytoplasma diagnostics. Bull Insectol 60:249–250Google Scholar
  2. 2.
    Nikolić P et al (2010) A panel of real-time PCR assays for specific detection of three phytoplasmas from the apple proliferation group. Mol Cell Probes 24:303–309PubMedCrossRefGoogle Scholar
  3. 3.
    Pirc M et al (2009) Improved fireblight diagnostics using quantitative real-time PCR detection of Erwinia amylovora chromosomal DNA. Plant Pathol 58:872–881CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Nataša Mehle
    • 1
    Email author
  • Petra Nikolić
    • 1
  • Matevž Rupar
    • 1
  • Jana Boben
    • 1
  • Maja Ravnikar
    • 1
  • Marina Dermastia
    • 1
  1. 1.National Institute of BiologyLjubljanaSlovenia

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