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Micropropagation of Citrus spp. by Organogenesis and Somatic Embryogenesis

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Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 994))

Abstract

Citrus spp., the largest fruit crops produced worldwide, are usually asexually propagated by cuttings or grafting onto seedling rootstocks. Most of Citrus genotypes are characterized by polyembryony due to the occurrence of adventive nucellar embryos, which lead to the production of true-to-type plants by seed germination. Tissue culture and micropropagation, in particular, are valuable alternatives to traditional propagation to obtain a high number of uniform and healthy plants in a short time and in a small space. Moreover, in vitro propagation provides a rapid system to multiply the progeny obtained by breeding programs, allows the use of monoembryonic and seedless genotypes as rootstocks, and it is very useful also for breeding and germplasm preservation.

In this chapter, two protocols regarding organogenesis of a rootstock and somatic embryogenesis of a cultivar have been described.

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Author information

Authors and Affiliations

  1. Dipartimento DEMETRA, Facoltà di Agraria, Università degli Studi di Palermo, Palermo, Italy

    Benedetta Chiancone & Maria Antonietta Germanà

Authors
  1. Benedetta Chiancone
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  2. Maria Antonietta Germanà
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Corresponding author

Correspondence to Maria Antonietta Germanà .

Editor information

Editors and Affiliations

  1. , IVALSA/Istituto per la Valorizzazione, National Research Council (CNR), Polo Scientifico, via Madonna del Piano n. 10, Sesto Fiorentino, Florence, 50019, Italy

    Maurizio Lambardi

  2. , Istituto per la Valorizzazione del Legno, National Research Council (CNR), n/a, Florence, n/a, Italy

    Elif Aylin Ozudogru

  3. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

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Chiancone, B., Germanà, M.A. (2012). Micropropagation of Citrus spp. by Organogenesis and Somatic Embryogenesis. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_8

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  • DOI: https://doi.org/10.1007/978-1-62703-074-8_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-073-1

  • Online ISBN: 978-1-62703-074-8

  • eBook Packages: Springer Protocols

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