Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Volume 994 of the series Methods in Molecular Biology pp 99-118


Micropropagation of Citrus spp. by Organogenesis and Somatic Embryogenesis

  • Benedetta ChianconeAffiliated withDipartimento DEMETRA, Facoltà di Agraria, Università degli Studi di Palermo
  • , Maria Antonietta GermanàAffiliated withDipartimento DEMETRA, Facoltà di Agraria, Università degli Studi di Palermo Email author 

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Citrus spp., the largest fruit crops produced worldwide, are usually asexually propagated by cuttings or grafting onto seedling rootstocks. Most of Citrus genotypes are characterized by polyembryony due to the occurrence of adventive nucellar embryos, which lead to the production of true-to-type plants by seed germination. Tissue culture and micropropagation, in particular, are valuable alternatives to traditional propagation to obtain a high number of uniform and healthy plants in a short time and in a small space. Moreover, in vitro propagation provides a rapid system to multiply the progeny obtained by breeding programs, allows the use of monoembryonic and seedless genotypes as rootstocks, and it is very useful also for breeding and germplasm preservation.

In this chapter, two protocols regarding organogenesis of a rootstock and somatic embryogenesis of a cultivar have been described.

Key words

Anther culture Citrus Epicotyl cuttings Organogenesis Somatic embryogenesis