Skip to main content

Part of the book series: Methods in Molecular Biology ((MIMB,volume 994))

Abstract

Persimmon (Diospyros kaki Thunb.) is a temperate fruit tree species diffused in all continents. The traditional propagation method adopted by the nursery industry is based on budding/grafting scion cultivars on seedlings from D. kaki, Diospyros lotus, and Diospyros virginiana, the most important species used as rootstock, reproduced by seeds since they are not easy to root. Furthermore, most of nonastringent cultivars of persimmon are not compatible with D. lotus, a rootstock largely utilized because of its hardiness and frost resistance. The main in vitro tissue culture techniques, developed for persimmon, deal with direct regeneration (from dormant buds and root tips), and indirect regeneration through callus from dormant buds, apexes, and leaves. The bottlenecks of micropropagation are (1) the recalcitrance of many cultivars to in vitro establishment, (2) the low multiplication ratio of D. kaki compared to other fruit tree species, (3) the very low rooting ability of ex novo microcuttings both from direct and indirect regeneration, (4) the high sensitivity to transplant from in vitro to in vivo conditions. The development of reliable in vitro regeneration procedures is likely to play a key role for production of both clonal rootstocks and self-rooted cultivars. The general protocol for micropropagation of persimmon reported here is based on the establishment of winter dormant buds in vitro, shoot development, multiplication and elongation, and shoot rooting, using cytokinins (BA or zeatin) in a MS media along with an auxinic pretreatment for rooting induction.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Yonemori K, Sugiura A, Yamada M (2000) Persimmon genetics and breeding. Plant Breed Rev 19:191–225

    Google Scholar 

  2. FAO (2011)—Faostat database; http://www.fao.org. Accessed 29 April 2011

  3. Yamada M (2008) Persimmon propagation, orchard planting, training and pruning in Japan. Adv Hortic Sci 4:269–273

    Google Scholar 

  4. Tao R, Sugiura A (1992) Micropropagation of Japanese persimmon (Diospyros kaki L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 18, High-tech and micropropagation 2. Springer, New York, pp 426–440

    Google Scholar 

  5. Yokoama T, Takeuchi M (1976) Organ and plantlet formation from callus in Japanese persimmon (Diospyros kaki). Phytomorphology 26:273–275

    Google Scholar 

  6. Yokoama T, Takeuchi M (1981) The induction and formation of organs in callus cultures from twigs of mature Japanese persimmon (Diospyros kaki Thunb.). J Jpn Soc Hortic Sci 49:557–562

    Google Scholar 

  7. Cooper PA, Cohen D (1984) Micropropagation of Japanese persimmon (Diospyros kaki). Comb Proc Int Plant Prop Soc 34:118–124

    Google Scholar 

  8. Fukui H, Sugiyama M, Nakamura M (1989) Shoot tip culture of Japanese persimmon (Diospyros kaki Thunb.). J Jpn Soc Hortic Sci 58:43–47

    Article  Google Scholar 

  9. Murashige T, Skoog F (1962) A revised medium for the rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–496

    Article  CAS  Google Scholar 

  10. Sugiura A, Tao R, Murayama H, Tomana T (1986) In vitro propagation of Japanese persimmon. Hortic Sci 21(5):1205–1207

    CAS  Google Scholar 

  11. Naval M, Llacer G, Badenes ML, Giordani E (2009) Adventitious shoot regeneration from leaf explants of the persimmon (Diospyros kaki Thunb.) cv. “Rojo Brillante”. Acta Hortic 833:183–186

    CAS  Google Scholar 

  12. Benelli C, Bellini E, Giordani E, Lambardi M (1999) In vitro plant regeneration and microprojectile bombardment of callus from Japanese persimmon shoot tips (Diospyros kaki L.f., cv Kaki Tipo). Adv Hortic Sci 13(2):82–86

    Google Scholar 

  13. Tao R, Dandekar AM, Uratsu SL, Vail PV, Tebbets JS (1997) Engineering genetic resistance against insects in Japanese persimmon using crylA(c) gene of Bacillus thuringiensis. J Am Soc Hortic Sci 122:764–771

    CAS  Google Scholar 

  14. Bellini E, Giordani E (1997) In vitro culture establishment and shoot elongation of Kaki Tipo (Diospyros kaki L.f.) dormant buds. Acta Hortic 436:129–134

    Google Scholar 

  15. Tetsumura T (1997) Effect of types of cytokinin used for in vitro shoot proliferation of Japanese persimmon on the subsequent rooting of shoots. Acta Hortic 436:143–148

    CAS  Google Scholar 

  16. Scaramucci S (2007) Studio sulla radicazione in vitro di Diospyros kaki L. Frutticoltura 12:52–58 [in Italian]

    Google Scholar 

  17. Tetsumura T, Koyanagi Y, Ito S, Habu T, Kawase K (2004) Comparative field performance of mature Japanese persimmon trees grafted on seedling rootstocks vs. micropropagated ones. J Jpn Soc Hortic Sci 73(2):134–136

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Edgardo Giordani .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media New York

About this protocol

Cite this protocol

Giordani, E., Naval, M., Benelli, C. (2012). In Vitro Propagation of Persimmon (Diospyros kaki Thunb.). In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_7

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-074-8_7

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-073-1

  • Online ISBN: 978-1-62703-074-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics