Micropropagation of Vaccinium sp. by In Vitro Axillary Shoot Proliferation

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 994)

Abstract

The Vaccinium genus contains several valuable fruit and ornamental species, among others: highbush blueberry (Vaccinium × corymbosum L.), cranberry (Vaccinium macrocarpon Ait.), and lingonberry (Vaccinium vitis-idaea L.). In some most popular and valuable cultivars, the conventional propagation methods, exploiting hard or soft wood cuttings, are inefficient. The demand for nursery plants could be fulfilled only by micropropagation. In principle cultivars are propagated in vitro through similar three-stage method, based on subculture of shoot explants on different culture media supplemented with IAA (0–4 mg/L) and 2iP (5–10 mg/L), and rooting shoots in vivo. The obtained plantlets are transferred to peat substrate and grown in the glasshouse until the end of growing period. The development of adventitious shoots should be monitored and controlled during in vitro stages. Many clones have specific requirements for growing conditions and/or are recalcitrant.

Key words

Adventitious shoots Axillary shoots Blueberry Cranberry Lingonberry Nodal explants 

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  1. 1.Faculty of Biology and Agriculture, Department of Plant Physiology and BiotechnologyUniversity of RzeszówRzeszówPoland

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