Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Volume 994 of the series Methods in Molecular Biology pp 279-289


Micropropagation of African Violet (Saintpaulia ionantha Wendl.)

  • Mukund ShuklaAffiliated withDepartment of Plant Agriculture, University of Guelph
  • , J. Alan SullivanAffiliated withDepartment of Plant Agriculture, University of Guelph
  • , Shri Mohan JainAffiliated withDepartment of Agricultural Sciences, University of Helsinki Email author 
  • , Susan J. MurchAffiliated withUniversity of British Columbia
  • , Praveen K. SaxenaAffiliated withDepartment of Plant Agriculture, University of Guelph Email author 

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Micropropagation is an important tool for rapid multiplication and the creation of genetic variability in African violets (Saintpaulia ionantha Wendl.). Successful in vitro propagation depends on the specific requirements and precise manipulation of various factors such as the type of explants used, physiological state of the mother plant, plant growth regulators in the culture medium, and growth conditions. Development of cost-effective protocols with a high rate of multiplication is a crucial requirement for commercial application of micropropagation. The current chapter describes an optimized protocol for micropropagation of African violets using leaf explants obtained from in vitro grown plants. In this process, plant regeneration occurs via both somatic embryogenesis and shoot organogenesis simultaneously in the explants induced with the growth regulator thidiazuron (TDZ; N-phenyl-N′-1,2,3-thidiazol-5-ylurea). The protocol is simple, rapid, and efficient for large-scale propagation of African violet and the dual routes of regeneration allow for multiple applications of the technology from simple clonal propagation to induction or selection of variants to the production of synthetic seeds.

Key words

African violet Saintpaulia ionantha Micropropagation Somatic embryogenesis Organogenesis Regeneration Root initiation Thidiazuron