Micropropagation of Pear (Pyrus sp.)

  • Barbara M. Reed
  • Jeanine DeNoma
  • Sugae Wada
  • Joseph Postman
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 994)

Abstract

Elements of micropropagation include establishment of shoot tip cultures, proliferation, rooting, and acclimatization of the resulting plantlets. The wide genetic variation in Pyrus makes micropropagation challenging for many genotypes. Initiation of shoots is most successful from forced dormant shoots or from scions grafted onto seedling rootstocks to impose juvenility. Clean shoots are recovered after testing for contaminants at the initiation stage on ½ strength Murashige and Skoog 1962 medium (MS), at pH 6.9 for 1 week or by streaking on nutrient agar. Although pear species and cultivars are cultured on several well-known media, MS is the most commonly used. Our studies showed that multiplication and growth of shoots are best on Pear Medium with higher concentrations of calcium chloride, potassium phosphate, and magnesium sulfate than MS medium and 4.4 μM N6 benzyladenine. Pear shoots are often recalcitrant to rooting; however, a 5 s dip in 10 mM indole-3-butyric acid or naphthalene acetic acid before planting on basal medium without plant growth regulators is effective for many genotypes. Pear shoots store well at 1–4°C, and can hold for as long as 4 years without reculture. Cryopreservation protocols are available for long-term storage of pear shoot tips. Acclimation of in vitro-rooted or micrografted shoots in a mist bed follows standard procedures.

Key words

Micrografting Micropropagation Mineral nutrition Multiplication Pyrus Shoot culture Rooting 

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Barbara M. Reed
    • 1
  • Jeanine DeNoma
    • 1
  • Sugae Wada
    • 2
  • Joseph Postman
    • 1
  1. 1.US Department of AgricultureAgricultural Research Service, National Clonal Germplasm RepositoryCorvallisUSA
  2. 2.Department of HorticultureOregon State UniversityCorvallisUSA

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