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Detection of Posttranslational Modifications of Replication Protein A

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Single-Stranded DNA Binding Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 922))

Abstract

Replication Protein A (RPA) is a single-strand DNA-binding protein that is found in all eukaryotes. RPA is subjected to multiple posttranslational modifications including serine- and threonine-phosphorylation, poly-ADP ribosylation, and SUMOylation. These modifications are believed to regulate RPA activity through modulating interactions with DNA and partner proteins. This article describes two methods used to detect posttranslational modified RPA: immunofluorescence and immmuoblotting.

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Acknowledgment

Thanks to all the current and past members of the Wold lab who have contributed to optimizing these protocols.

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Authors and Affiliations

  1. Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, USA

    Cathy S. Hass, Ran Chen & Marc S. Wold

Authors
  1. Cathy S. Hass
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  2. Ran Chen
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  3. Marc S. Wold
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Corresponding author

Correspondence to Marc S. Wold .

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Editors and Affiliations

  1. School of Medicine and Public Health, Department of Biomolecular Chemistry, University of Wisconsin, Henry Mall 420, Madison, 53706, USA

    James L. Keck

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© 2012 Springer Science+Business Media, LLC

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Hass, C.S., Chen, R., Wold, M.S. (2012). Detection of Posttranslational Modifications of Replication Protein A. In: Keck, J. (eds) Single-Stranded DNA Binding Proteins. Methods in Molecular Biology, vol 922. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-032-8_15

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  • DOI: https://doi.org/10.1007/978-1-62703-032-8_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-031-1

  • Online ISBN: 978-1-62703-032-8

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