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Malaria pp 567-576 | Cite as

Vaccination Using Normal Live Sporozoites Under Drug Treatment

  • Laurent RéniaEmail author
  • Anne Charlotte Grüner
  • Marjorie Mauduit
  • Georges Snounou
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 923)

Abstract

Sterile immunity against malaria has been obtained in mammalian hosts exclusively through vaccination with whole parasite preparations. Induction of complete protection against challenge was obtained using sporozoites attenuated by irradiation or genetic manipulations. It has been demonstrated recently that immunization with normal sporozoites under chloroquine cover confers sterile protection in mice and humans, using substantially fewer parasites and injections than with irradiated sporozoite immunization. Subsequently, it was shown that other drugs can substitute for chloroquine. We describe the immunization protocol using live sporozoites under chloroquine cover, which confers sterile immunity in rodents.

Key words

Malaria Sporozoites Vaccine Parasite Plasmodium Drug 

Notes

Acknowledgments

This work was supported by intramural grants from the Agency for Science, technology, and Research (A*STAR) and Institut National de la Sante et de la Recherche Medicale (INSERM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Laurent Rénia
    • 1
    Email author
  • Anne Charlotte Grüner
    • 1
  • Marjorie Mauduit
    • 1
  • Georges Snounou
    • 2
  1. 1.Singapore Immunology Network, Agency for Science, Technology and Research, BiopolisSingaporeSingapore
  2. 2.Hôpital Pitié-Salpêtrière, Université Paris 6ParisFrance

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