Malaria pp 493-504 | Cite as

Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination

  • Katherine L. Doll
  • Noah S. Butler
  • John T. HartyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 923)


CD8 T cells are critical mediators of protection against Plasmodium liver-stage infection. Most studies of the CD8 T cell response to whole parasite Plasmodium vaccines address a single T cell epitope in BALB/c mice, and thus provide limited information. Here, we describe a surrogate activation marker approach that uses the coordinate downregulation of the CD8α chain and upregulation of the integrin CD11a to track the total CD8 T cell response to Plasmodium vaccination via flow cytometry. With this approach, quantitative (magnitude, kinetics) and qualitative (distribution, phenotype, and function) features of the total CD8 T cell response to vaccination with attenuated Plasmodium or other pathogens can be studied.

Key words

Plasmodium CD8 T cell Malaria Radiation-attenuated sporozoite Vaccine 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Katherine L. Doll
    • 1
  • Noah S. Butler
    • 1
  • John T. Harty
    • 2
    Email author
  1. 1.Department of MicrobiologyUniversity of IowaIowa CityUSA
  2. 2.Department of Microbiology, Pathology and ImmunologyUniversity of IowaIowa CityUSA

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